Evidence map›Paper›PMID 38597436›Full record

ArticleNan fang yi ke da xue xue bao = Journal of Southern Medical University2024

[High expression of UBE2S promotes progression of hepatocellular carcinoma by increasing cancer cell stemness].

H Chen, Z Li, M Wang, L Lu, Q Tang, L Luo

Open access · greenAbstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 0 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

H ChenPostdoctoral Research Station of Clinical Medicine, Jinan University, Guangzhou 510632, China.
Z LiSchool of Clinical Medicine, Wannan Medical College, Wuhu 241002, China.
M WangDepartment of Obstetrics, Nanjing First Hospital, Nanjing 210006, China.
L LuDepartment of Pathology, Wannan Medical College, Wuhu 241002, China.
Q TangGraduate School, Youjiang Medical University for Nationalities, Baise 533000, China.
L LuoPostdoctoral Research Station of Clinical Medicine, Jinan University, Guangzhou 510632, China.
Wannan Medical College · CNAffiliated Hospital of Youjiang Medical University for Nationalities · CNJinan University · CNNanjing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the expression of the ubiquitination enzyme UBE2S in different cell types in hepatocellular carcinoma (HCC) microenvironment and its impact on proliferation and stemness of HCC cells.

methodsTCGA and CPTAC database were used to analyze the transcriptional and promoter methylation levels and protein expressions of UBE2S in HCC. Specific expression patterns of UBE2S, intercellular communication and key transcription factors in different cell types were analyzed based on single-cell sequencing data from TISCH website. We further examined UBE2S expressions in clinical samples of HCC tissues, HCC cells and T cells using immunohistochemistry and immunofluorescence staining. We also tested the effects of UBE2S knockdown on stemness of HCC-LM3 and HepG2 cells using clone formation experiments and sphere formation assay.

resultsAnalysis based on TCGA database suggested significant overexpression of UBE2S in both paired and non-paired tumor tissues (

conclusionUBE2S is highly expressed in T cells in HCC microenvironment in close correlation with a poor prognosis. High UBE2S expression promotes the stemness of HCC cells.

Indexed as

Carcinoma, HepatocellularLiver NeoplasmsCell Line, TumorEndothelial CellsGene Expression Regulation, NeoplasticHep G2 CellsHumansPrognosisTumor MicroenvironmentUbiquitin-Conjugating EnzymesUbe2S protein, humanUbiquitin-Conjugating Enzymeshepatocellular carcinomasingle-cell sequencingT cellsUBE2S

Identifiers

PMID38597436
PMCPMC11006698
OpenAlexW4394738688

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