Evidence map›Paper›PMID 38595673›Full record

ArticleVeterinary world2024

Use of cytobrush for bacteriological and cytological diagnosis of endometritis in mares.

Chiara Del Prete, Francesca Paola Nocera, Giuseppe Piegari, Veronica Palumbo, Luisa De Martino, Natascia Cocchia, Orlando Paciello, Chiara Montano, Maria Pia Pasolini

Open access · goldAbstract read
In one paragraph

Article in Veterinary world, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.3field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Chiara Del PreteReproduction Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Francesca Paola NoceraDiagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Giuseppe PiegariDiagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Veronica PalumboRegional Reference Center for Urban Veterinary Hygiene (CRIUV), Naples, Italy.
Luisa De MartinoDiagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Natascia CocchiaReproduction Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Orlando PacielloDiagnostic Service of Pathology and Animal Health, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Chiara MontanoSurgery Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Maria Pia PasoliniSurgery Unit, Department of Veterinary Medicine and Animal Productions, University of Naples Federico II, Naples, Italy.
Federico II University Hospital · ITUniversity of Naples Federico II · ITInstitute for Urban and Regional Research · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and Aim: A combined microbial and cytological examination of uterine samples is the main diagnostic method for endometritis in mares. This study aimed to describe a procedure for using the same uterine cytobrush (CB) for both bacteriological and cytological evaluation. Material and Methods: The procedure consists of rolling the CB onto a sterilized glass slide immediately after collection and before the transfer into a sterile saline solution. In Experiment 1, a comparison between bacteriological results of the cotton swab (CS) and CB or pellet was made in 10 mares; in Experiment 2, bacteriological and cytological results were compared between different processing methods of CB in 28 mares; in other 6 mares, a CB was processed for cytology only, to investigate the reasons for the low cellularity of the pellet. Results: The agreement between culture results from the CB and CS was evaluated, and a comparison between the cytological data obtained by different processing methods of CB was performed. The perfect agreement between the CB and CS microbiological results was found. The described procedure enables useful diagnostic smears for cytology. Moreover, the seeding of both the tip of CB and the saline solution used for the transport produced accurate bacteriological results. Conclusion: The protocol described in this study for the use of CB for both cytological and bacteriological analysis could be used for the diagnosis of endometritis. To maximize diagnostic sample quality, cytology slides must be prepared with meticulous care in the field to preserve cellular integrity and minimize artifacts.

Indexed as

endometritisfield conditionsmaremicrobiology brushuterine sampling

Identifiers

PMID38595673
PMCPMC11000473
OpenAlexW4391888582

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.