Evidence map›Paper›PMID 38595329›Full record

ArticleBioMed research international2024

First Molecular Detection of SARS-CoV-2 in Sewage and Wastewater in Ghana.

Ewurabena Oduma Duker, Evangeline Obodai, Seth Offei Addo, Lorreta Kwasah, Edna Serwah Mensah, Emmanuel Gberbi, Abraham Anane, Keren O Attiku, Jessica Boakye, Gayheart Deladem Agbotse and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Environmental surveillance of pathogens in Africa.Applied and environmental microbiology · 2026
    Review
  3. Article
  4. Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool.International journal of molecular sciences · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Ewurabena Oduma DukerVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Evangeline ObodaiVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.ORCID https://orcid.org/0000-0001-5358-5688
Seth Offei AddoParasitology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.ORCID https://orcid.org/0000-0003-4470-4504
Lorreta KwasahVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Edna Serwah MensahVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Emmanuel GberbiVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Abraham AnaneVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Keren O AttikuVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Jessica BoakyeVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Gayheart Deladem AgbotseVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Angelina Evelyn DicksonVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Joseph Ahia QuarcooVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Patience Akosua DarkoVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Yaw Awuku LarbiVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.ORCID https://orcid.org/0000-0003-0265-6639
Nana Afia Asante NtimVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.
Bartholomew DzudzorDepartment of Medical Biochemistry, University of Ghana Medical School, University of Ghana, Legon, Accra, Ghana.
John Kofi OdoomVirology Department, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon, Accra, Ghana.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is shed in the stool of infected individuals and can be detected in sewage and wastewater contaminated with infected stool. This study is aimed at detecting the virus and its potential survival in sewage and wastewater in Ghana. The cross-sectional study included samples from 16 validated environmental surveillance sites in 7 regions of Ghana. A total of 354 samples composed of wastewater (280) and sewage (74) were collected from November 2020 to November 2022. Overall, 17% of the samples were positive for SARS-CoV-2 by real-time PCR, with 6% in sewage and 11% in wastewater. The highest number of positive samples was collected from the Greater Accra Region (7.3%) with the least recorded in the Bono East Region (0.6%). Further characterization of the positive samples using the next-generation sequencing (NGS) approach yielded two variants: Alpha (B.1.1.7) and Delta (AY.36). Attempts to isolate SARS-CoV-2 in the Vero cell line were not successful probably due to the low viral load concentrations (Ct values > 35) or prolonged exposure to high temperatures rendering the virus noninfectious. Our findings suggest that SARS-CoV-2 RNA in sewage and wastewater may not be infectious, but the prevalence shows that the virus persists in the communities within Ghana.

Indexed as

COVID-19SewageCross-Sectional StudiesGhanaHumansRNA, ViralSARS-CoV-2WastewaterRNA, ViralSewageWastewater

Identifiers

PMID38595329
PMCPMC11003379

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.