Evidence map›Paper›PMID 38594538›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Chromatin Immunoprecipitation to Investigate H2A.Z Dynamics in Response to Environmental Changes.

J Paola Saldierna Guzmán, Mark Zander, Björn C Willige

Erratum issuedAbstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 89% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

J Paola Saldierna GuzmánDepartment of Soil and Crop Sciences, College of Agricultural Sciences, Colorado State University, Fort Collins, CO, USA.
Mark ZanderWaksman Institute of Microbiology, Department of Plant Biology, School of Environmental and Biological Sciences, Rutgers, The State University of New Jersey, Piscataway, NJ, USA. mzander@waksman.rutgers.edu.
Björn C WilligeDepartment of Soil and Crop Sciences, College of Agricultural Sciences, Colorado State University, Fort Collins, CO, USA. bjorn.willige@colostate.edu.
Colorado State University · USRutgers, The State University of New Jersey · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA methylation and posttranslational modifications of histones instruct gene expression in eukaryotes. Besides canonical histones, histone variants also play a critical role in transcriptional regulation. One of the best studied histone variants in plants is H2A.Z whose removal from gene bodies correlates with increased transcriptional activity. The eviction of H2A.Z is regulated by environmental cues such as increased ambient temperatures, and current models suggest that H2A.Z functions as a transcriptional buffer preventing environmentally responsive genes from undesired activation. To monitor temperature-dependent H2A.Z dynamics, chromatin immunoprecipitation (ChIP) of H2A.Z-occupied DNA can be performed. The following protocol describes a quick and easy ChIP approach to study in vivo H2A.Z occupancy.

Indexed as

Gene Expression RegulationHistonesChromatinChromatin ImmunoprecipitationDNA MethylationNucleosomesTemperatureChromatinHistonesNucleosomesChromatinChromatin immunoprecipitationH2A.ZHistonesThermomorphogenesis

Identifiers

PMID38594538
OpenAlexW4394599130

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.