Analysis of Phase Separation of EARLY FLOWERING 3.

Maolin Peng, Stephanie Hutin, Aleksandra Mironova, Chloe Zubieta, Philip A Wigge

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

3.0field-weighted citation impact, top 12% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 2 countries.

Maolin PengLeibniz-Institut für Gemüse-und Zierpflanzenbau, Theodor-Echtermeyer-Weg 1, Großbeeren, Germany.

Stephanie HutinLaboratoire de Physiologie Cellulaire and Végétale, Univ. Grenoble Alpes/CNRS/CEA/INRA/IRIG, Grenoble, France.

Aleksandra MironovaLaboratoire de Physiologie Cellulaire and Végétale, Univ. Grenoble Alpes/CNRS/CEA/INRA/IRIG, Grenoble, France.

Chloe ZubietaLaboratoire de Physiologie Cellulaire and Végétale, Univ. Grenoble Alpes/CNRS/CEA/INRA/IRIG, Grenoble, France.

Philip A WiggeLeibniz-Institut für Gemüse-und Zierpflanzenbau, Theodor-Echtermeyer-Weg 1, Großbeeren, Germany. wigge@igzev.de.

Leibniz Institute of Vegetable and Ornamental Crops · DECEA Grenoble · FRLaboratoire de Physiologie CellulaireLaboratoire Physiologie Cellulaire & Végétale · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Phase separation is an important mechanism for regulating various cellular functions. The EARLY FLOWERING 3 (ELF3) protein, an essential element of the EVENING COMPLEX (EC) involved in circadian clock regulation, has been shown to undergo phase separation. ELF3 is known to significantly influence elongation growth and flowering time regulation, and this is postulated to be due to whether the protein is in the dilute or phase-separated state. Here, we present a brief overview of methods for analyzing ELF3 phase separation in vitro, including the generation of phase diagrams as a function of pH and salt versus protein concentrations, optical microscopy, fluorescence recovery after photobleaching (FRAP), and turbidity assays.

Indexed as

ArabidopsisArabidopsis ProteinsCircadian ClocksCircadian RhythmGene Expression Regulation, PlantLightMutationPhase SeparationTranscription FactorsArabidopsis ProteinsTranscription FactorsEARLY FLOWERING 3 (ELF3)FRAPPhase diagramsPhase separationPrion-like domainTurbidity assays

Identifiers

PMID38594534

DOI10.1093/nar/gky508

OpenAlexW4394599152

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.