Evidence map›Paper›PMID 38592604›Full record

ArticleBiomedical microdevices2024

Automated sample preparation for electrospray ionization mass spectrometry based on CLOCK-controlled autonomous centrifugal microfluidics.

Masahiro Futami, Hiroki Naito, Satoshi Ninomiya, Lee Chuin Chen, Tomohiko Iwano, Kentaro Yoshimura, Yoshiaki Ukita

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical microdevices, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 57% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Masahiro FutamiDepartment of Engineering, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, Graduate School of University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan.
Hiroki NaitoDepartment of Engineering, Integrated Graduate School of Medicine, Engineering, and Agricultural Sciences, Graduate School of University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan.
Satoshi NinomiyaGraduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan.
Lee Chuin ChenGraduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan.
Tomohiko IwanoDepartment of Anatomy and Cell Biology, Faculty of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, 409-3898, Japan.
Kentaro YoshimuraDivision of Molecular Biology, Center for Medical Education and Sciences, Interdisciplinary Graduate School of Medicine, University of Yamanashi, 1110 Shimokato, Chuo, 409-3898, Japan.
Yoshiaki UkitaGraduate Faculty of Interdisciplinary Research, University of Yamanashi, 4-3-11 Takeda, Kofu, 400-8510, Japan. yukita@yamanashi.ac.jp.
University of Yamanashi · JPTakeda (Japan) · JPUniversity of Yamanashi Hospital · JP

Funding

Japan Keirin Autorace Foundation #2021M135.
6 · The paper itself

Abstract

We report a centrifugal microfluidic device that automatically performs sample preparation under steady-state rotation for clinical applications using mass spectrometry. The autonomous microfluidic device was designed for the control of liquid operation on centrifugal hydrokinetics (CLOCK) paradigm. The reported device was highly stable, with less than 7% variation with respect to the time of each unit operation (sample extraction, mixing, and supernatant extraction) in the preparation process. An agitation mechanism with bubbling was used to mix the sample and organic solvent in this device. We confirmed that the device effectively removed the protein aggregates from the sample, and the performance was comparable to those of conventional manual sample preparation procedures that use high-speed centrifugation. In addition, probe electrospray ionization mass spectrometry (PESI-MS) was performed to compare the device-treated and manually treated samples. The obtained PESI-MS spectra were analyzed by partial least squares discriminant analysis, and the preparation capability of the device was found to be equivalent to that of the conventional method.

Indexed as

MicrofluidicsSpectrometry, Mass, Electrospray IonizationCentrifugationLab-On-A-Chip DevicesRotationAutonomous microfluidic controlCentrifugal microfluidicsMass spectrometrySample preparation

Identifiers

PMID38592604
PMCPMC11003918
OpenAlexW4394615213

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.