Evidence map›Paper›PMID 38590133›Full record

ArticleJournal of clinical laboratory analysis2024

A Multiplex Recombinase-Aided qPCR Assay for Highly Sensitive and Rapid Detection of khe, bla

Shao-Wei Hua, Jie Wang, Zi-Jin Zhao, Feng-Yu Tian, Meng Zhao, Yu-Xin Wang, Rui-Qing Zhang, Zhi-Qiang Han, Shi-Jue Gao, Xiao-Na Lv and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Detection ofPathogens (Basel, Switzerland) · 2025
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Shao-Wei HuaGraduate School, Hebei North University, Zhangjiakou, China.
Jie WangDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang, China.
Zi-Jin ZhaoGraduate School, Hebei North University, Zhangjiakou, China.
Feng-Yu TianNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Meng ZhaoNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Yu-Xin WangNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Rui-Qing ZhangNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Zhi-Qiang HanNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Shi-Jue GaoNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Xiao-Na LvGraduate School, Hebei North University, Zhangjiakou, China.
Hong-Yi LiGraduate School, Hebei North University, Zhangjiakou, China.
Xin-Xin ShenNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.
Xue-Jun MaNational Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases, NHC Key Laboratory of Medical Virology and Viral Diseases, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China.ORCID https://orcid.org/0000-0001-5891-5260
Zhi-Shan FengDepartment of Clinical Laboratory, Hebei General Hospital, Shijiazhuang, China.

Funding

National Key R&D Program of China 2021YFC2301102National Natural Science Foundation of China 82202593
6 · The paper itself

Abstract

objectiveThis study aimed to establish a highly sensitive and rapid single-tube, two-stage, multiplex recombinase-aided qPCR (mRAP) assay to specifically detect the khe, bla

methodsmRAP was carried out in a qPCR instrument within 1 h. The analytical sensitivities of mRAP for khe, bla

resultsmRAP achieved the sensitivities of 10, 8, and 14 copies/reaction for khe, bla

conclusionmRAP is a rapid and highly sensitive assay for potential clinical identification of khe, bla

Indexed as

beta-LactamasesKlebsiella pneumoniaeMultiplex Polymerase Chain ReactionBacterial ProteinsHumansKlebsiella InfectionsReal-Time Polymerase Chain ReactionRecombinasesSensitivity and SpecificityBacterial Proteinsbeta-lactamase NDM-1beta-LactamasesRecombinasesblaKPC‐2blaNDM‐1Carbapenem‐resistant Klebsiella pneumoniae (CRKP)kheRAA

Identifiers

PMID38590133
PMCPMC11137842

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.