ArticleStem cell research & therapy2024
Oral ulcer treatment using human tonsil-derived mesenchymal stem cells encapsulated in trimethyl chitosan hydrogel: an animal model study.
Article in Stem cell research & therapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 11 citations in OpenAlex.
- Differential Wound Healing Potential of Tonsil Parenchymal and Epithelial Mesenchymal Stromal Cell-Derived Exosomes.Tissue engineering and regenerative medicine · 2026Article
- Mesenchymal Stem Cell Therapy for Oral Lichen Planus: A Paradigm Shift from Palliation to Regeneration.International journal of hematology-oncology and stem cell research · 2026Article
- Evolution of 3D-Printed Microneedles toward Closed-Loop Theranostic Platforms.Research (Washington, D.C.) · 2026Review
- Human periodontal ligament stem cells promote oral ulcer healing in rats through modulation of TGF-β1/smad signaling.Frontiers in cell and developmental biology · 2026Article
- Stem Cell and Exosome Therapy in Wound Healing: Traps, Paradoxes, and Tricks Transforming Paradigms.Biomedicines · 2025Article
- Oxidized Hyaluronic Acid-Based Sponges: A Promising Biomaterial for Oral Mucosa Lesion Application.International journal of molecular sciences · 2025Article
- Bionic Microneedle Patch Inspired by Drosophila Tarsal Paws Boosts Healing in Bacterial Infectious Stomatitis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A cell-free SHED lysate-hydrogel system for oral ulcer healing with anti-inflammatory and pro-angiogenic effects.Journal of nanobiotechnology · 2025Article
- Evaluation of Preclinical Efficacy of Curcumin-Loaded Bicosome Systems in Amelioration of Oral Mucositis.Pharmaceutics · 2025Article
- Oral stem cells in combination with hydrogels as biomimetic bioactive platforms for periodontal tissue engineering.Frontiers in oral health · 2025Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
backgroundOral ulcers are a common side effect of chemotherapy and affect patients' quality of life. While stem cell transplantation is a potential treatment for oral ulcers, its efficacy is limited as the stem cells tend to remain in the affected area for a short time. This study aims to develop a treatment for oral ulcers by using trimethyl chitosan (TMC) hydrogel with human tonsil-derived stem cells (hTMSCs) to increase the therapeutic effect of stem cells and investigate their effectiveness.
methodsAnimals were divided into four experimental groups: Control, TMC hydrogel, hTMSCs, and hTMSCs loaded in TMC hydrogel (Hydrogel + hTMSCs) (each n = 8). Oral ulcers were chemically induced by anesthetizing the rats followed by injection of dilute acetic acid in the right buccal mucosa. After confirming the presence of oral ulcers in the animals, a single subcutaneous injection of 100 µL of each treatment was applied to the ulcer area. Histological analyses were performed to measure inflammatory cells, oral mucosal thickness, and fibrosis levels. The expression level of inflammatory cytokines was also measured using RT-PCR to gauge therapeutic the effect.
resultsThe ulcer size was significantly reduced in the TMC hydrogel + hTMSCs group compared to the control group. The stem cells in the tissue were only observed until Day 3 in the hTMSCs treated group, while the injected stem cells in the TMC Hydrogel + hTMSCs group were still present until day 7. Cytokine analysis related to the inflammatory response in the tissue confirmed that the TMC Hydrogel + hTMSCs treated group demonstrated superior wound healing compared to other experimental groups.
conclusionThis study has shown that the adhesion and viability of current stem cell therapies can be resolved by utilizing a hydrogel prepared with TMC and combining it with hTMSCs. The combined treatment can promote rapid healing of oral cavity wounds by enhancing anti-inflammatory effects and expediting wound healing. Therefore, hTMSC loaded in TMC hydrogel was the most effective wound-healing approach among all four treatment groups prolonging stem cell survival. However, further research is necessary to minimize the initial inflammatory response of biomaterials and assess the safety and long-term effects for potential clinical applications.
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