Evidence map›Paper›PMID 38589895›Full record

ArticleJournal of nanobiotechnology2024

Alkylated EDTA potentiates antibacterial photodynamic activity of protoporphyrin.

Ying Piao, Sebastian Himbert, Zifan Li, Jun Liu, Zhihao Zhao, Huahai Yu, Shuangshuang Liu, Shiqun Shao, Michael Fefer, Maikel C Rheinstädter and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Ying PiaoZhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Sebastian HimbertDepartment of Physics and Astronomy, McMaster University, Hamilton, ON, L8S 3Z5, Canada. himberts@mcmaster.ca.
Zifan LiZhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Jun LiuSuncor AgroScience, Mississauga, ON, L5K 1A8, Canada.
Zhihao ZhaoZhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Huahai YuZhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Shuangshuang LiuZhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China.
Shiqun ShaoZhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China. shiqun.shao@zju.edu.cn.
Michael FeferSuncor AgroScience, Mississauga, ON, L5K 1A8, Canada.
Maikel C RheinstädterDepartment of Physics and Astronomy, McMaster University, Hamilton, ON, L8S 3Z5, Canada. rheinstadter@mcmaster.ca.
Youqing ShenZhejiang Key Laboratory of Smart Biomaterials and Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310058, China. shenyq@zju.edu.cn.
Zhejiang University · CNMcMaster University · CASuncor Energy (Canada) · CAMinistry of Education · NZ

Funding

Basic Public Welfare Research Program of Zhejiang Province LGC21B040001Fundamental Research Funds for the Central Universities 226-2023-00022Key Research and Development Program of Zhejiang Province 2020C01123National Key Research and Development Program of China 2021YFA1201200National Natural Science Foundation of China 51833008Natural Sciences and Engineering Research Council of Canada ALLRP 570723 - 21
6 · The paper itself

Abstract

Antibiotic resistance has garnered significant attention due to the scarcity of new antibiotics in development. Protoporphyrin IX (PpIX)-mediated photodynamic therapy shows promise as a novel antibacterial strategy, serving as an alternative to antibiotics. However, the poor solubility of PpIX and its tendency to aggregate greatly hinder its photodynamic efficacy. In this study, we demonstrate that alkylated EDTA derivatives (aEDTA), particularly C14-EDTA, can enhance the solubility of PpIX by facilitating its dispersion in aqueous solutions. The combination of C14-EDTA and PpIX exhibits potent antibacterial activity against Staphylococcus aureus (S. aureus) when exposed to LED light irradiation. Furthermore, this combination effectively eradicates S. aureus biofilms, which are known to be strongly resistant to antibiotics, and demonstrates high therapeutic efficacy in an animal model of infected ulcers. Mechanistic studies reveal that C14-EDTA can disrupt PpIX crystallization, increase bacterial membrane permeability and sequester divalent cations, thereby improving the accumulation of PpIX in bacteria. This, in turn, enhances reactive oxygen species (ROS) production and the antibacterial photodynamic activity. Overall, this effective strategy holds great promise in combating antibiotic-resistant strains.

Indexed as

PhotochemotherapyStaphylococcus aureusAnimalsAnti-Bacterial AgentsEdetic AcidPhotosensitizing AgentsProtoporphyrinsAnti-Bacterial AgentsEdetic AcidPhotosensitizing Agentsprotoporphyrin IXProtoporphyrins

Identifiers

PMID38589895
PMCPMC11003131
OpenAlexW4394582307

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.