Evidence map›Paper›PMID 38586335›Full record

ArticleHeliyon2024

Biological and molecular characterization of citrus bent leaf viroid.

Mustansar Mubeen, Faheema Bakhtawar, Yasir Iftikhar, Qaiser Shakeel, Ashara Sajid, Rashid Iqbal, Reem M Aljowaie, Talha Chaudhary

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mustansar MubeenDepartment of Plant Pathology, College of Agriculture, University of Sargodha, Sargodha, 40100, Pakistan.
Faheema BakhtawarPest Warning & Quality Control of Pesticides, Sargodha, 40100, Pakistan.
Yasir IftikharDepartment of Plant Pathology, College of Agriculture, University of Sargodha, Sargodha, 40100, Pakistan.
Qaiser ShakeelCholistan Institute of Desert Studies, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.
Ashara SajidDepartment of Plant Pathology, College of Agriculture, University of Sargodha, Sargodha, 40100, Pakistan.
Rashid IqbalDepartment of Agronomy, Faculty of Agriculture and Environment, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan.
Reem M AljowaieDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. 2455, Riyadh, 11451, Saudi Arabia.
Talha ChaudharyFaculty of Agricultural and Environmental Sciences, Hungarian University of Agriculture and Life Sciences 2100, Godollo, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background and aim: Citrus bent leaf viroid (CBLVd) is one of the emerging and widely distributed viroids in citrus-growing areas of the world, including Pakistan. Previously, CBLVd has been reported in Pakistan for the first time in 2009. Therefore, characterization of CBLVd is required to monitor the viroid status in the citrus orchards concerning citrus decline. Methods: Biological and molecular characterization of CBLVd was studied through biological indexing and confirmation through RT-PCR, followed by phylogenetic analysis of selected CBLVd isolates. Among four citrus cultivars viz., Kinnow ( Results: Symptoms such as mild mosaic, slight backward leaf bending, and leaf curling were observed after eight months' post-inoculation. Conclusions: There was slight genetic variability, but more than 90% relevance was found among the submitted and already reported CBLVd isolate from Pakistan. Scanty literature is available regarding the biological and molecular studies of CBLVd in Pakistan. Therefore, the transmission and molecular characterization of CBLVd in Pakistan were studied for the first time.

Indexed as

CBLVdCitrus speciesHost rangeRT-PCRTransmission

Identifiers

PMID38586335
PMCPMC10998147

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.