Evidence map›Paper›PMID 38586058›Full record

ArticlebioRxiv : the preprint server for biology2024

Regenerative human liver organoids (HLOs) in a pillar/perfusion plate for hepatotoxicity assays.

Sunil Shrestha, Prabha Acharya, Soo-Yeon Kang, Manav Goud Vanga, Vinod Kumar Reddy Lekkala, Jiafeng Liu, Yong Yang, Pranav Joshi, Moo-Yeal Lee

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 6 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Sunil ShresthaDepartment of Biomedical Engineering, University of North Texas, Denton, Texas, USA.
Prabha AcharyaDepartment of Biomedical Engineering, University of North Texas, Denton, Texas, USA.
Soo-Yeon KangDepartment of Biomedical Engineering, University of North Texas, Denton, Texas, USA.
Manav Goud VangaBioprinting Laboratories Inc., Dallas, Texas, USA.
Vinod Kumar Reddy LekkalaDepartment of Biomedical Engineering, University of North Texas, Denton, Texas, USA.
Jiafeng LiuDepartment of Biomedical Engineering, University of North Texas, Denton, Texas, USA.
Yong YangDepartment of Biomedical Engineering, University of North Texas, Denton, Texas, USA.
Pranav JoshiBioprinting Laboratories Inc., Dallas, Texas, USA.
Moo-Yeal LeeDepartment of Biomedical Engineering, University of North Texas, Denton, Texas, USA.ORCID 0000-0001-6922-609X
University of North Texas · US

Funding

Modeling diabetes using an integrated plate systemUH3DK119982 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI LEE, MOO-YEAL, WELLS, JAMES M · 2020 to 2022
$5.4M
Pillar and perfusion well plate platforms for reproducible organoid culture from iPSCR44TR003491 · NCATS · BIOPRINTING LABORATORIES, INC. · PI JOSHI, PRANAV, LEE, MOO-YEAL · 2020 to 2021
$838k
Gene-edited liver organoids for predictive hepatotoxicityR43ES035653 · NIEHS · BIOPRINTING LABORATORIES, INC. · PI JOSHI, PRANAV, LEE, MOO-YEAL · 2023 to 2023
$285k
NCATS NIH HHS R44 TR003491NIDDK NIH HHS UH3 DK119982NIEHS NIH HHS R43 ES035653
6 · The paper itself

Abstract

Human liver organoids (HLOs) differentiated from embryonic stem cells (ESCs), induced pluripotent stem cells (iPSCs), and adult stem cells (ASCs) can recapitulate the structure and function of human fetal liver tissues, thus being considered as a promising tissue model for liver diseases and predictive compound screening. However, the adoption of HLOs in drug discovery faces several technical challenges, which include the lengthy differentiation process with multiple culture media leading to batch-to-batch variation, short-term maintenance of hepatic functions post-maturation, low assay throughput due to Matrigel dissociation and HLO transfer to a microtiter well plate, and insufficient maturity levels compared to primary hepatocytes. To address these issues, expandable HLOs (Exp-HLOs) derived from human iPSCs were generated by optimizing differentiation protocols, which were rapidly printed on a 144-pillar plate with sidewalls and slits (144PillarPlate) and dynamically cultured for up to 20 days into differentiated HLOs (Diff-HLOs) in a 144-perfusion plate with perfusion wells and reservoirs (144PerfusionPlate) for

Indexed as

dynamic organoid culturehepatotoxicity testingorganoid bioprintingperfusion platepillar plateRegenerative human liver organoids

Identifiers

PMID38586058
PMCPMC10996672
OpenAlexW4393307501

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.