ArticlebioRxiv : the preprint server for biology2024
Sensitive Fluorescent Biosensor Reveals Differential Subcellular Regulation of PKC.
Qi Su, Jing Zhang, Wei Lin, Jin-Fan Zhang, Alexandra C Newton, Sohum Mehta, Jing Yang, Jin Zhang
Open access · greenAbstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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0citing papers in PubMed
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What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
0 citing papers in PubMed, 5 citations in OpenAlex.
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4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
8 authors at 1 institution in 1 country.
Qi SuDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA, USA.ORCID 0000-0002-7393-1696 Jing ZhangDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA, USA.
Wei LinDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA, USA.ORCID 0000-0002-2128-241X Jin-Fan ZhangShu Chien-Gene Lay Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.
Alexandra C NewtonDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA, USA.ORCID 0000-0002-2906-3705 Sohum MehtaDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA, USA.ORCID 0000-0003-4764-8579 Jing YangDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA, USA.
Jin ZhangDepartment of Pharmacology, School of Medicine, University of California San Diego, La Jolla, CA, USA.
University of California San Diego · US
Funding
Live-cell Activity Architecture in CancerR35CA197622 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jin Zhang · 2015 to 2026
$11.2MMolecular Mechanisms of Cell SignalingR35GM122523 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ALEXANDRA C. NEWTON · 2017 to 2026
$6.7MRegulation of Tumor Invasion and Metastasis by Matrix StiffnessR01CA174869 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jing Yang · 2015 to 2026
$4.3MUltrasensitive kinase biosensors for multiplex imaging of coordinated spatiotemporal signaling in cancer-immune interactionsR01CA262815 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yingxiao Wang, Jin Zhang · 2022 to 2026
$3.3MEPHA2 Receptor Signaling in Breast Cancer MechanotransductionR01CA262794 · NCI · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI ELENA B PASQUALE, Jing Yang · 2022 to 2026
$2.9MDevelopment of kinase biosensors for multiplex neuronal imaging of signaling pathways in behaving miceRF1MH126707 · NIMH · JOHNS HOPKINS UNIVERSITY · PI HUGANIR, RICHARD L, ZHANG, JIN · 2022 to 2022
$2.4MApical-basal polarity in tumor progression and metastasisR01CA236386 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI YANG, JING · 2019 to 2023
$1.9MNCI NIH HHS R01 CA174869NCI NIH HHS R01 CA236386NCI NIH HHS R01 CA262794NCI NIH HHS R01 CA262815NCI NIH HHS R35 CA197622NIGMS NIH HHS R35 GM122523NIMH NIH HHS RF1 MH126707
6 · The paper itselfAbstract
The protein kinase C (PKC) family of serine/threonine kinases, which consist of three distinctly regulated subfamilies, have long been established as critical for a variety of cellular functions. However, how PKC enzymes are regulated at different subcellular locations, particularly at emerging signaling hubs such as the ER, lysosome, and Par signaling complexes, is unclear. Here, we present a sensitive Excitation Ratiometric (ExRai) C Kinase Activity Reporter (ExRai-CKAR2) that enables the detection of minute changes in subcellular PKC activity. Using ExRai-CKAR2 in conjunction with an enhanced diacylglycerol (DAG) biosensor capable of detecting intracellular DAG dynamics, we uncover the differential regulation of PKC isoforms at distinct subcellular locations. We find that G-protein coupled receptor (GPCR) stimulation triggers sustained PKC activity at the ER and lysosomes, primarily mediated by Ca
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PMID38586003
PMCPMC10996667
OpenAlexW4393355295
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