Evidence map›Paper›PMID 38585828›Full record

ArticlebioRxiv : the preprint server for biology2024

Sequencing Strategy to Ensure Accurate Plasmid Assembly.

Sarah I Hernandez, Casey-Tyler Berezin, Katie M Miller, Samuel J Peccoud, Jean Peccoud

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Sarah I HernandezDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado, 80523, United States of America.
Casey-Tyler BerezinDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado, 80523, United States of America.
Katie M MillerDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado, 80523, United States of America.
Samuel J PeccoudDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado, 80523, United States of America.
Jean PeccoudDepartment of Chemical and Biological Engineering, Colorado State University, Fort Collins, Colorado, 80523, United States of America.
Colorado State University · US

Funding

Supplement: Development of a technology to certify engineered DNA moleculesR01GM147816 · NIGMS · COLORADO STATE UNIVERSITY · PI PECCOUD, JEAN M · 2022 to 2024
$1.4M
Leveraging cytoplasmic transcription to develop self-amplifying DNA vaccinesR21AI168482 · NIAID · COLORADO STATE UNIVERSITY · PI PECCOUD, JEAN M · 2023 to 2024
$400k
NIAID NIH HHS R21 AI168482NIGMS NIH HHS R01 GM147816
6 · The paper itself

Abstract

Despite the wide use of plasmids in research and clinical production, the need to verify plasmid sequences is a bottleneck that is too often underestimated in the manufacturing process. Although sequencing platforms continue to improve, the method and assembly pipeline chosen still influence the final plasmid assembly sequence. Furthermore, few dedicated tools exist for plasmid assembly, especially for

Identifiers

PMID38585828
PMCPMC10996661
OpenAlexW4393231112

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.