Evidence map›Paper›PMID 38585750›Full record

ArticlebioRxiv : the preprint server for biology2024

Effectiveness of fixation methods for wholemount immunohistochemistry across cellular compartments in chick embryos.

Camilo V Echeverria, Tess A Leathers, Crystal D Rogers

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Camilo V EcheverriaDepartment of Anatomy, Physiology, and Cell Biology, University of California, Davis, Davis, CA, USA.
Tess A LeathersDepartment of Anatomy, Physiology, and Cell Biology, University of California, Davis, Davis, CA, USA.
Crystal D RogersDepartment of Anatomy, Physiology, and Cell Biology, University of California, Davis, Davis, CA, USA.ORCID 0000-0002-9549-1089

Funding

UC Davis MCB T32 Administrative Supplement to Recognize Excellence in Diversity, Equity, Inclusion, and Accessibility (DEIA) MentorshipT32GM007377 · NIGMS · UNIVERSITY OF CALIFORNIA DAVIS · PI CHEDIN, FREDERIC LOUIS · 1985 to 2023
$9.9M
Mechanisms of microtubule-mediated cranial neural crest EMT and differentiationR03DE032047 · NIDCR · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ROGERS, CRYSTAL D · 2022 to 2023
$300k
NIDCR NIH HHS R03 DE032047NIGMS NIH HHS T32 GM007377
6 · The paper itself

Abstract

The choice of fixation method significantly impacts tissue morphology and protein visualization after immunohistochemistry (IHC). In this study, we compared the effects of paraformaldehyde (PFA) and trichloroacetic acid (TCA) fixation prior to IHC on chicken embryos. Our findings underscore the importance of validating fixation methods for accurate interpretation of IHC results, with implications for antibody validation and tissue-specific protein localization studies. We found that TCA fixation resulted in larger and more circular nuclei compared to PFA fixation. Additionally, TCA fixation altered the appearance of subcellular localization and fluorescence intensity of various proteins, including transcription factors and cytoskeletal proteins. Notably, TCA fixation revealed protein localization domains that may be inaccessible with PFA fixation. These results highlight the need for optimization of fixation protocols depending on the target epitope and model system, emphasizing the importance of methodological considerations in biological analyses.

Indexed as

cadherinchickenimmunohistochemistryneural crestparaformaldehydePaxSoxtrichloracetic acidtubulin

Identifiers

PMID38585750
PMCPMC10996528

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.