ArticleAnalytical methods : advancing methods and applications2024
An on-line SPE-LC-MS/MS method for quantification of nucleobases and nucleosides present in biological fluids.
Article in Analytical methods : advancing methods and applications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 5 citations in OpenAlex.
- ZIF-8 based on plasmonic metal organic framework hybrid gold nanoparticles for ultra-sensitive and multi-channel SERS detection of urinary metabolites.Mikrochimica acta · 2026Article
- Ultraperformance Liquid Chromatography Tandem Mass Spectrometry Assay of DNA Cytosine Methylation Excretion from Biological Systems.ACS omega · 2025Article
- Comparative Analysis of Metabolites of Wild and CultivatedMolecules (Basel, Switzerland) · 2025Article
- Preparation of sulfonic acid functionalized metal organic frameworks and their application in the online solid phase extraction of parabens and sulfonamides in pre-cooked foods.Food chemistry: X · 2025Article
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Solid phase extraction (SPE) and liquid chromatographic (LC) separation of nucleobases and nucleosides are challenging due to the high hydrophilicity of these compounds. Herein we report a novel on-line SPE-LC-MS/MS method for their quantification after pre-column derivatization with chloroacetaldehyde (CAA). The method proposed is selective and sensitive with limits of detection at the nano-molar level. Analysis of urine and saliva samples by using this method is demonstrated. Adenine, guanine, cytosine, adenosine, guanosine, and cytidine were found in the range from 0.19 (guanosine) to 1.83 μM (cytidine) in urine and from 0.015 (guanosine) to 0.79 μM (adenine) in saliva. Interestingly, methylation of cytidine was found to be significantly different in urine from that in saliva. While 5-hydroxymethylcytidine was detected at a very low level (<0.05 μM) in saliva, it was found to be the most prominent methylated cytidine in urine at a high level of 3.33 μM. Since on-line SPE is deployed, the proposed LC-MS/MS quantitative assay is convenient to carry out and offers good assay accuracy and repeatability.
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