Evidence map›Paper›PMID 38584223›Full record

ArticleGastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association2024

Genome-wide 5-hydroxymethylcytosines in circulating cell-free DNA as noninvasive diagnostic markers for gastric cancer.

Yingli Fu, Jing Jiang, Yanhua Wu, Donghui Cao, Zhifang Jia, Yangyu Zhang, Dongming Li, Yingnan Cui, Yuzheng Zhang, Xueyuan Cao

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Article in Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

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0cells of the map it votes in
10citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Yingli Fu *Division of Clinical Epidemiology, The First Hospital of Jilin University, Changchun, China.
Jing Jiang *Division of Clinical Epidemiology, The First Hospital of Jilin University, Changchun, China.
Yanhua WuDivision of Clinical Epidemiology, The First Hospital of Jilin University, Changchun, China.
Donghui CaoDivision of Clinical Epidemiology, The First Hospital of Jilin University, Changchun, China.
Zhifang JiaDivision of Clinical Epidemiology, The First Hospital of Jilin University, Changchun, China.
Yangyu ZhangDepartment of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.
Dongming LiDepartment of Hospital Infection Management, China-Japan Union Hospital of Jilin University, Changchun, China.
Yingnan CuiDepartment of Hospital Infection Management, China-Japan Union Hospital of Jilin University, Changchun, China.
Yuzheng ZhangDepartment of Epidemiology and Biostatistics, School of Public Health, Jilin University, Changchun, China.
Xueyuan CaoDepartment of Gastric and Colorectal Surgery, General Surgery Center, The First Hospital of Jilin University, Changchun, China. jd3d2ub@jlu.edu.cn.ORCID 0000-0002-1982-1672

Funding

Department of Finance of Jilin Province JLSWSRCZX2021-073National Natural Science Foundation of China 81874279National Natural Science Foundation of China 82373664Natural Science Foundation of Jilin Province YDZJ202201ZYTS064
6 · The paper itself

Abstract

background5-Hydroxymethylcytosine-enriched gene profiles and regions show tissue-specific and tumor specific. There is a potential value to explore cell-free DNA 5-hydroxymethylcytosine feature biomarkers for early gastric cancer detection.

methodsA matched case‒control study design with 50 gastric cancer patients and 50 controls was performed to sequence the different 5-hydroxymethylcytosine modification features of cell free DNA. Significantly differential 5-hydroxymethylcytosine modification genes were identified to construct a gastric cancer diagnostic model. Data set from GEO was used as an external testing set to test the robustness of the diagnostic model.

resultsAccounting for more than 90% of 5-hydroxymethylcytosine peaks were distributed in the gene body in both the gastric cancer and control groups. The diagnostic model was developed based on five different 5-hydroxymethylcytosine modification genes, FBXL7, PDE3A, TPO, SNTG2 and STXBP5. The model could effectively distinguish gastric cancer patients from controls in the training (AUC = 0.95, sensitivity = 88.6%, specificity = 94.3%), validation (AUC = 0.87, sensitivity = 73.3%, specificity = 93.3%) and testing (AUC = 0.90, sensitivity = 81.9%, specificity = 90.2%) sets. The risk scores of the controls from the model were significantly lower than those of gastric cancer patients in both our own data (P < 0.001) and GEO external testing data (P < 0.001), and no significant difference between different TNM stage patients (P = 0.09 and 0.66). Furthermore, there was no significant difference between the healthy control and benign gastric disease patients in the testing set from GEO (P = 0.10).

conclusionsThe characteristics of 5-hydroxymethylcytosine in cell free DNA are specific to gastric cancer patients, and the diagnostic model constructed by five genes' 5-hydroxymethylcytosine features could effectively identify gastric cancer patients.

Indexed as

5-MethylcytosineBiomarkers, TumorCell-Free Nucleic AcidsStomach NeoplasmsAgedCase-Control StudiesDNA MethylationEarly Detection of CancerFemaleHumansMaleMiddle Aged5-hydroxymethylcytosine5-MethylcytosineBiomarkers, TumorCell-Free Nucleic Acids5-HydroxymethylcytosineCell-free DNAGastric cancer

Identifiers

PMID38584223

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.