Evidence map›Paper›PMID 38583808›Full record

ArticleJournal of virological methods2024

Insertion of foreign genes into the simian varicella virus genome by Tn7-mediated site-specific transposition.

Wayne L Gray

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 96% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Wayne L GrayBiology Department, University of Mississippi, MS 38677, USA. Electronic address: wlgray@olemiss.edu.
University of Mississippi · US

Funding

Tracking Glycosylation State with Fluorinated Glycosides ProbesP20GM130460 · NIGMS · UNIVERSITY OF MISSISSIPPI · PI Joshua S Sharp · 2020 to 2026
$17.9M
Animal models to design &evaluate improved VZV vaccinesR01AI052373 · NIAID · UNIV OF ARKANSAS FOR MED SCIS · PI GRAY, WAYNE L · 2003 to 2007
$1.5M
Laser spectroscopic instrumentation for isotopic sensing of biogenic Nitric OxideR21GM103460 · NIGMS · PRINCETON UNIVERSITY · PI WYSOCKI, GERARD · 2012 to 2012
$190k
NIAID NIH HHS R01 AI052373NIGMS NIH HHS P20 GM130460NIGMS NIH HHS R21 GM103460
6 · The paper itself

Abstract

A Tn7-transposition approach was utilized for site-specific insertion of foreign genes into the genome of simian varicella virus (SVV), the causative agent of simian varicella in nonhuman primates. The severe acute respiratory syndrome coronavirus (SARS-CoV-2) nucleocapsid (N) gene and receptor binding domain (RBD) of the spike gene were inserted into the ORF 14 region of the SVV genome cloned into a bacterial artificial chromosome and then transfected into Vero cells to generate the infectious recombinant SVV (rSVV). The rSVV replicated efficiently in infected Vero cells and expressed the N and RBD antigens as indicated by immunoblot and immunofluorescence assays. Tn7-mediated transposition provides a rapid and efficient method for constructing rSVVs which may be evaluated as live-attenuated vaccines.

Indexed as

Genome, ViralAnimalsChlorocebus aethiopsChromosomes, Artificial, BacterialDNA Transposable ElementsMutagenesis, InsertionalNucleocapsid ProteinsSARS-CoV-2Spike Glycoprotein, CoronavirusVaricellovirusVero CellsVirus ReplicationDNA Transposable ElementsNucleocapsid ProteinsSpike Glycoprotein, CoronavirusSevere acute respiratory syndrome coronavirus (SARS-CoV-2)Simian varicella virusTn7 transposition

Identifiers

PMID38583808
PMCPMC11129925
OpenAlexW4394010045

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.