Evidence map›Paper›PMID 38583365›Full record

ArticleBiomaterials2024

Engineered extracellular vesicle-based gene therapy for the treatment of discogenic back pain.

Shirley N Tang, Ana I Salazar-Puerta, Mary K Heimann, Kyle Kuchynsky, María A Rincon-Benavides, Mia Kordowski, Gilian Gunsch, Lucy Bodine, Khady Diop, Connor Gantt and 9 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
8.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 20 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
  4. Isolation of Extracellular Vesicles From Mouse Brown Adipose Tissue Secretome Using Size-Exclusion Chromatography.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2026
    Article
  5. Review
  6. Article
  7. Review
  8. Review
  9. Review
  10. Article
  11. Rewiring cell identityNanomedicine (London, England) · 2025
    Article
  12. Article
  13. Editorial: Women in science: musculoskeletal pain.Frontiers in pain research (Lausanne, Switzerland) · 2025
    Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

19 authors at 7 institutions in 2 countries.

Shirley N TangDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA.
Ana I Salazar-PuertaDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA.
Mary K HeimannDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA.
Kyle KuchynskyDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA.
María A Rincon-BenavidesBiophysics Graduate Program, The Ohio State University, USA.
Mia KordowskiBiophysics Graduate Program, The Ohio State University, USA.
Gilian GunschDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA.
Lucy BodineDepartment of Mechanical Engineering, College of Engineering, The Ohio State University, USA.
Khady DiopDepartment of Biology, College of Arts and Sciences, The Ohio State University, USA.
Connor GanttDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA.
Safdar KhanDepartment of Orthopedics, The Ohio State University Wexner Medical Center, USA.
Anna BrataszSmall Animal Imaging Center Shared Resources, Wexner Medical Center, USA.
Olga Kokiko-CochranDepartment of Neuroscience, The Ohio State University, USA; Institute for Behavioral Medicine Research, Neurological Institute, The Ohio State University, USA.
Julie FitzgeraldDepartment of Neuroscience, The Ohio State University, USA; Institute for Behavioral Medicine Research, Neurological Institute, The Ohio State University, USA.
Damien M LaudierLeni and Peter W. May Department of Orthopaedics, Icahn School of Medicine at Mount Sinai, USA.
Judith A HoylandDivision of Cell Matrix Biology and Regenerative Medicine, School of Biological Sciences, The University of Manchester, Manchester, UK; NIHR Manchester Musculoskeletal Biomedical Research Centre, Manchester University, NHS Foundation Trust, Manchester Academic Health Science Centre, Manchester, UK.
Benjamin A WalterDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA; Department of Orthopedics, The Ohio State University Wexner Medical Center, USA.
Natalia Higuita-CastroDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA; Biophysics Graduate Program, The Ohio State University, USA; Department of Neurosurgery, The Ohio State University, USA; Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, USA. Electronic address: Higuitacastro.1@osu.edu.
Devina PurmessurDepartment of Biomedical Engineering, College of Engineering, The Ohio State University, USA; Department of Orthopedics, The Ohio State University Wexner Medical Center, USA. Electronic address: Purmessurwalter.1@osu.edu.
The Ohio State University · USThe Ohio State University Wexner Medical Center · USInstitute for Behavioral Medicine · USFlorida State University · USIcahn School of Medicine at Mount Sinai · USLung Institute · USManchester Academic Health Science Centre · GB

Funding

Novel non-viral reprogramming strategies to treat Discogenic back pain via engineered extracellular vesiclesR01AR079485 · NIAMS · OHIO STATE UNIVERSITY · PI Natalia Higuita-Castro, Devina Purmessur (Walter) · 2022 to 2026
$2.5M
Non-viral Reprogramming of Intervertebral Disc Cells for the treatment of Discogenic back painR61AR076786 · NIAMS · OHIO STATE UNIVERSITY · PI HIGUITA-CASTRO, NATALIA, PURMESSUR (WALTER), DEVINA · 2019 to 2019
$368k
NIAMS NIH HHS R01 AR079485NIAMS NIH HHS R61 AR076786
6 · The paper itself

Abstract

Painful musculoskeletal disorders such as intervertebral disc (IVD) degeneration associated with chronic low back pain (termed "Discogenic back pain", DBP), are a significant socio-economic burden worldwide and contribute to the growing opioid crisis. Yet there are very few if any successful interventions that can restore the tissue's structure and function while also addressing the symptomatic pain. Here we have developed a novel non-viral gene therapy, using engineered extracellular vesicles (eEVs) to deliver the developmental transcription factor FOXF1 to the degenerated IVD in an in vivo model. Injured IVDs treated with eEVs loaded with FOXF1 demonstrated robust sex-specific reductions in pain behaviors compared to control groups. Furthermore, significant restoration of IVD structure and function in animals treated with FOXF1 eEVs were observed, with significant increases in disc height, tissue hydration, proteoglycan content, and mechanical properties. This is the first study to successfully restore tissue function while modulating pain behaviors in an animal model of DBP using eEV-based non-viral delivery of transcription factor genes. Such a strategy can be readily translated to other painful musculoskeletal disorders.

Indexed as

Extracellular VesiclesGenetic TherapyIntervertebral Disc DegenerationAnimalsBack PainFemaleForkhead Transcription FactorsIntervertebral DiscLow Back PainMaleRats, Sprague-DawleyForkhead Transcription FactorsCell reprogrammingEngineered extracellular vesiclesIntervertebral discLow back painNanocarriersNon-viral gene delivery

Identifiers

PMID38583365
PMCPMC11164054
OpenAlexW4393378828

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.