Evidence map›Paper›PMID 38580844›Full record

ArticleNature methods2024

Selective-plane-activation structured illumination microscopy.

Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Architecture-driven quantitative nanoscopy maps cytoskeleton remodeling.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  11. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Kenta Temma *Department of Applied Physics, Osaka University, Osaka, Japan.ORCID http://orcid.org/0000-0002-9968-3459
Ryosuke Oketani *Department of Applied Physics, Osaka University, Osaka, Japan.
Toshiki KuboDepartment of Applied Physics, Osaka University, Osaka, Japan.ORCID http://orcid.org/0000-0002-5696-4111
Kazuki BandoDepartment of Applied Physics, Osaka University, Osaka, Japan.
Shunsuke MaedaDepartment of Applied Physics, Osaka University, Osaka, Japan.
Kazunori SugiuraSANKEN (The Institute of Scientific and Industrial Research), Osaka University, Osaka, Japan.
Tomoki MatsudaSANKEN (The Institute of Scientific and Industrial Research), Osaka University, Osaka, Japan.ORCID http://orcid.org/0000-0002-1906-8406
Rainer HeintzmannLeibniz Institute of Photonic Technology, Jena, Germany.ORCID http://orcid.org/0000-0002-4950-1936
Tatsuya KaminishiInstitute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan.ORCID http://orcid.org/0000-0001-5056-1074
Koki FukudaInstitute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan.
Maho HamasakiDepartment of Genetics, Graduate School of Medicine, Osaka University, Osaka, Japan.
Takeharu NagaiInstitute for Open and Transdisciplinary Research Initiatives, Osaka University, Osaka, Japan.ORCID http://orcid.org/0000-0003-2650-9895
Katsumasa FujitaDepartment of Applied Physics, Osaka University, Osaka, Japan. fujita@ap.eng.osaka-u.ac.jp.ORCID http://orcid.org/0000-0002-2284-375X

Funding

MEXT | JST | Center of Innovation Program (COI) JPMJPF2009MEXT | JST | Core Research for Evolutional Science and Technology (CREST) JPMJCR15N3MEXT | JST | Core Research for Evolutional Science and Technology (CREST) JPMJCR1925Ministry of Education, Culture, Sports, Science and Technology (MEXT) 18H05410
6 · The paper itself

Abstract

The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.

Indexed as

Microscopy, FluorescenceGreen Fluorescent ProteinsHumansLightingLuminescent ProteinsSpheroids, CellularGreen Fluorescent ProteinsLuminescent Proteins

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.