ArticleThe EMBO journal2024
Limited oxygen in standard cell culture alters metabolism and function of differentiated cells.
Article in The EMBO journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 40 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
40 citing papers in PubMed, 47 citations in OpenAlex.
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- Longitudinal Monitoring of Metabolic Gradients in Microreactor Culture Platforms by Raman Spectroscopy.Biosensors · 2026Article
- Peptide hormones and bile acids shaping immune tolerance of the liver: implications and applications.Protein & cell · 2026Review
- Integrated analysis of the adipocyte plasma membrane proteome reveals KCC1 and PIT2 as novel insulin-responsive transporters.The Journal of biological chemistry · 2026Article
- Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo.Nature methods · 2026Article
- Engineering biomimetic tissue barrier models on chips: From design and fabrication to applications in disease modeling and drug screening.Biomaterials · 2026Review
- Dynamic subcellular proteomics identifies regulators of adipocyte insulin action.Nature communications · 2026Article
- GABA Receptor Activation in Müller Glia as a Molecular Switch for Controlling VEGF-A in the Retina.ASN neuro · 2026Article
- Adjusting PSC culture for neural organoid generation.Stem cell reports · 2026Article
- A novel culture flask for clinostat-based simulation of extraterrestrial gravities.Frontiers in cell and developmental biology · 2026Article
- A feedback loop between cell proliferation and ROS regulates ferroptosis sensitivity.Frontiers in cell and developmental biology · 2026Article
- Oxygen Handling Practices for in Vitro Human Pluripotent Stem Cell Cardiomyocyte Differentiation: A Scoping Literature Review.Stem cell reviews and reports · 2026Article
- Oxygen-enhanced assembloid-based vascularized intestinal-on-a-chip for radioprotective drug evaluation.Frontiers in toxicology · 2026Article
- Article
- WhenAPL bioengineering · 2025Review
Corrections and comments
- Erratum issued
Authors and funding
30 authors at 5 institutions in 5 countries.
Funding
Abstract
The in vitro oxygen microenvironment profoundly affects the capacity of cell cultures to model physiological and pathophysiological states. Cell culture is often considered to be hyperoxic, but pericellular oxygen levels, which are affected by oxygen diffusivity and consumption, are rarely reported. Here, we provide evidence that several cell types in culture actually experience local hypoxia, with important implications for cell metabolism and function. We focused initially on adipocytes, as adipose tissue hypoxia is frequently observed in obesity and precedes diminished adipocyte function. Under standard conditions, cultured adipocytes are highly glycolytic and exhibit a transcriptional profile indicative of physiological hypoxia. Increasing pericellular oxygen diverted glucose flux toward mitochondria, lowered HIF1α activity, and resulted in widespread transcriptional rewiring. Functionally, adipocytes increased adipokine secretion and sensitivity to insulin and lipolytic stimuli, recapitulating a healthier adipocyte model. The functional benefits of increasing pericellular oxygen were also observed in macrophages, hPSC-derived hepatocytes and cardiac organoids. Our findings demonstrate that oxygen is limiting in many terminally-differentiated cell types, and that considering pericellular oxygen improves the quality, reproducibility and translatability of culture models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.