Evidence map›Paper›PMID 38579711›Full record

ArticleStem cell reports2024

MAGIK: A rapid and efficient method to create lineage-specific reporters in human pluripotent stem cells.

Tahir Haideri, Jirong Lin, Xiaoping Bao, Xiaojun Lance Lian

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 46% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. CRISPR Gene Tagging for Illuminating Endogenous Protein Dynamics.International journal of molecular sciences · 2026
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Tahir HaideriDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Jirong LinDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Xiaoping BaoDavidson School of Chemical Engineering, Purdue University, West Lafayette, IN 47907, USA. Electronic address: bao61@purdue.edu.
Xiaojun Lance LianDepartment of Biomedical Engineering, Pennsylvania State University, University Park, PA 16802, USA; Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, PA 16802, USA; Department of Biology, Pennsylvania State University, University Park, PA 16802, USA. Electronic address: lian@psu.edu.
Pennsylvania State University · USPurdue University West Lafayette · US

Funding

Engineer Biomimetic Microfluidic Models to Investigate and Reprogram Tumor Associated Neutrophils for Cancer TherapyR37CA265926 · NCI · PURDUE UNIVERSITY · PI Xiaoping Bao · 2022 to 2026
$1.7M
Stem cell immunoengineering for universal cardiac therapy via CRISPR-Cas9R21EB026035 · NIBIB · PENNSYLVANIA STATE UNIVERSITY, THE · PI LIAN, XIAOJUN · 2018 to 2020
$567k
ModRNA-based Direct Programming of Universal Donor hiPSCs into Immune Evasive Beta CellsR56DK133147 · NIDDK · PENNSYLVANIA STATE UNIVERSITY, THE · PI LIAN, XIAOJUN · 2023 to 2024
$465k
NCI NIH HHS R37 CA265926NIBIB NIH HHS R21 EB026035NIDDK NIH HHS R56 DK133147
6 · The paper itself

Abstract

Precise insertion of fluorescent proteins into lineage-specific genes in human pluripotent stem cells (hPSCs) presents challenges due to low knockin efficiency and difficulties in isolating targeted cells. To overcome these hurdles, we present the modified mRNA (ModRNA)-based Activation for Gene Insertion and Knockin (MAGIK) method. MAGIK operates in two steps: first, it uses a Cas9-2A-p53DD modRNA with a mini-donor plasmid (without a drug selection cassette) to significantly enhance efficiency. Second, a deactivated Cas9 activator modRNA and a 'dead' guide RNA are used to temporarily activate the targeted gene, allowing for live cell sorting of targeted cells. Consequently, MAGIK eliminates the need for drug selection cassettes or labor-intensive single-cell colony screening, expediting precise gene editing. We showed MAGIK can be utilized to insert fluorescent proteins into various genes, including SOX17, NKX6.1, NKX2.5, and PDX1, across multiple hPSC lines. This underscores its robust performance and offers a promising solution for achieving knockin in hPSCs within a significantly shortened time frame.

Indexed as

Cell LineageGene Knock-In TechniquesPluripotent Stem CellsCell LineCRISPR-Cas SystemsGene EditingGenes, ReporterHumansRNA, Guide, CRISPR-Cas SystemsRNA, MessengerRNA, Guide, CRISPR-Cas SystemsRNA, MessengerCRISPRgene activationgene editinglineage-specific reportersMAGIKmodified mRNApluripotent stem cells

Identifiers

PMID38579711
PMCPMC11103783
OpenAlexW4393932552

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.