Evidence map›Paper›PMID 38578576›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Characterization of Bitter Taste Receptor-Dependent Autophagy in Oral Epithelial Cells.

Nisha Singh, Saeid Ghavami, Prashen Chelikani

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
4.0field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Nisha SinghManitoba Chemosensory Biology (MCSB) Research Group, Winnipeg, MB, Canada.
Saeid GhavamiResearch Institute of Oncology and Hematology, Cancer Care Manitoba-University of Manitoba, Winnipeg, MB, Canada. Saeid.ghavami@umanitoba.ca.
Prashen ChelikaniManitoba Chemosensory Biology (MCSB) Research Group, Winnipeg, MB, Canada. Prashen.Chelikani@umanitoba.ca.
CancerCare Manitoba · CAResearch Manitoba · CAUniversity of Manitoba · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbial dysbiosis is an important trigger in the development of oral diseases. Oral keratinocytes or gingival epithelial cells (GECs) offer protection against various microbial insults. Recent studies suggest that GECs expressed higher level of bitter taste receptor 14 (T2R14) compared to other taste receptors and toll-like receptors and act as innate immune sentinels. Macroautophagy or autophagy is a cellular conserved process involved in the regulation of host innate immune responses against microbial infection. Here, we describe a robust method for evaluation of T2R14-dependent autophagy flux in GECs. Autophagy flux was detected using Western blot analysis in GECs and further was confirmed using Acridine Orange-dependent flow cytometry analysis.

Indexed as

AutophagyEpithelial CellsMouth MucosaReceptors, G-Protein-CoupledCells, CulturedFlow CytometryGingivaHumansImmunity, InnateKeratinocytesReceptors, G-Protein-CoupledAcridine OrangeAutophagosomeAutophagy flux inhibitionBitter taste receptorFlow cytometryOral epithelial cellsSerum starvationWestern blot

Identifiers

PMID38578576
OpenAlexW4393978647

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.