Evidence map›Paper›PMID 38577628›Full record

ArticleNeurophotonics2024

Blue-shifted genetically encoded Ca

Abhi Aggarwal, Smrithi Sunil, Imane Bendifallah, Michael Moon, Mikhail Drobizhev, Landon Zarowny, Jihong Zheng, Sheng-Yi Wu, Alexander W Lohman, Alison G Tebo and 4 more

Open access · goldAbstract read
In one paragraph

Article in Neurophotonics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
3.3field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors at 7 institutions in 3 countries.

Abhi AggarwalUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.ORCID https://orcid.org/0000-0002-6336-566X
Smrithi SunilAllen Institute for Neural Dynamics, Seattle, Washington, United States.
Imane BendifallahSorbonne University, Vision Institute, CNRS, INSERM, Paris, France.ORCID https://orcid.org/0000-0003-4576-0903
Michael MoonUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Mikhail DrobizhevMontana State University, Department of Microbiology and Cell Biology, Bozeman, Montana, United States.
Landon ZarownyUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Jihong ZhengHoward Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, United States.
Sheng-Yi WuUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Alexander W LohmanUniversity of Calgary, Hotchkiss Brain Institute, Department of Cell Biology and Anatomy Calgary, Alberta, Canada.
Alison G TeboHoward Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia, United States.ORCID https://orcid.org/0000-0003-0788-5617
Valentina EmilianiSorbonne University, Vision Institute, CNRS, INSERM, Paris, France.ORCID https://orcid.org/0000-0003-2992-9510
Kaspar PodgorskiAllen Institute for Neural Dynamics, Seattle, Washington, United States.
Yi ShenUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.
Robert E CampbellUniversity of Alberta, Department of Chemistry, Edmonton, Alberta, Canada.ORCID https://orcid.org/0000-0003-0604-092X
University of Alberta · CAHoward Hughes Medical Institute · USInserm · FRAllen Institute · USJanelia Research Campus · USMontana State University · USUniversity of Calgary · CA

Funding

Resource for Multiphoton Characterization of Genetically-Encoded ProbesU24NS109107 · NINDS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Mikhail Drobizhev · 2018 to 2026
$2.2M
NINDS NIH HHS U24 NS109107
6 · The paper itself

Abstract

Significance: Genetically encoded calcium ion ( Aim: We describe the development and applications of T-GECO1-a high-performance blue-shifted GECI based on the Approach: We use protein engineering and extensive directed evolution to develop T-GECO1. We characterize the purified protein and assess its performance Results: The Conclusions: T-GECO1 is a high-performance blue-shifted GECI that, under two-photon excitation conditions, provides advantages relative to late generation GCaMP variants.

Indexed as

blue-shifted fluorescencegenetically encoded calcium ion indicatorneuronal activity imagingprotein engineeringtwo-photon excitation

Identifiers

PMID38577628
PMCPMC10993905
OpenAlexW4393950251

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.