Evidence map›Paper›PMID 38576723›Full record

ArticleRSC chemical biology2024

Development of PCR primers enabling the design of flexible sticky ends for efficient concatenation of long DNA fragments.

Kohei Nomura, Kaoru Onda, Hirotaka Murase, Fumitaka Hashiya, Yukiteru Ono, Goro Terai, Natsuhisa Oka, Kiyoshi Asai, Daisuke Suzuki, Naho Takahashi and 6 more

Abstract read
In one paragraph

Article in RSC chemical biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Kohei NomuraDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.
Kaoru OndaDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.
Hirotaka MuraseDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.
Fumitaka HashiyaResearch Center for Materials Science, Nagoya University Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan.
Yukiteru OnoDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo Kashiwanoha, Kashiwa Chiba 277-8561 Japan.
Goro TeraiDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo Kashiwanoha, Kashiwa Chiba 277-8561 Japan.
Natsuhisa OkaDepartment of Chemistry and Biomolecular Science Faculty of Engineering, Gifu University Gifu 501-1193 Japan.ORCID https://orcid.org/0000-0002-3114-9233
Kiyoshi AsaiDepartment of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo Kashiwanoha, Kashiwa Chiba 277-8561 Japan.
Daisuke SuzukiDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.
Naho TakahashiDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.
Haruka HiraokaDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0002-8022-6186
Masahito InagakiDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.
Yasuaki KimuraDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0002-3609-602X
Yoshihiro ShimizuLaboratory for Cell-Free Protein Synthesis, RIKEN Center for Biosystems Dynamics Research Suita Osaka 565-0874 Japan.
Naoko AbeDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.
Hiroshi AbeDepartment of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku Nagoya Aichi 464-8602 Japan h-abe@chem.nagoya-u.ac.jp.ORCID https://orcid.org/0000-0003-0048-3789

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed chemically modified PCR primers that allow the design of flexible sticky ends by introducing a photo-cleavable group at the phosphate moiety. Nucleic acid derivatives containing

Identifiers

PMID38576723
PMCPMC10989509

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.