Evidence map›Paper›PMID 38575766›Full record

ArticleJournal of medical ultrasonics (2001)2024

Facilitatory effect of low-pulse repetition frequency ultrasound on release of extracellular vesicles from cultured myotubes.

Xiaoqi Ma, Atomu Yamaguchi, Noriaki Maeshige, Kento Tanida, Mikiko Uemura, Fuwen Lu, Hiroyo Kondo, Hidemi Fujino

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medical ultrasonics (2001), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Xiaoqi MaDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, 10-2 Tomogaoka 7-chome, Suma-ku, Kobe, Hyogo, 654-0142, Japan.
Atomu YamaguchiDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, 10-2 Tomogaoka 7-chome, Suma-ku, Kobe, Hyogo, 654-0142, Japan.
Noriaki MaeshigeDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, 10-2 Tomogaoka 7-chome, Suma-ku, Kobe, Hyogo, 654-0142, Japan. nmaeshige@pearl.kobe-u.ac.jp.ORCID http://orcid.org/0000-0002-3573-347X
Kento TanidaDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, 10-2 Tomogaoka 7-chome, Suma-ku, Kobe, Hyogo, 654-0142, Japan.
Mikiko UemuraDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, 10-2 Tomogaoka 7-chome, Suma-ku, Kobe, Hyogo, 654-0142, Japan.
Fuwen LuShanghai Yangzhi Rehabilitation Hospital (Shanghai Sunshine Rehabilitation Center), Tongji University School of Medicine, 2209 Guangxing Rd., Songjiang District, Shanghai, 201619, China.
Hiroyo KondoFaculty of Health and Nutrition, Shubun University, 72 Momo Higashiyashiki, Yamato-cho, Ichinomiya, Aichi, 491-0932, Japan.
Hidemi FujinoDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences, 10-2 Tomogaoka 7-chome, Suma-ku, Kobe, Hyogo, 654-0142, Japan.
Kobe University · JPHyogo University of Health Sciences · JPShanghai Sunshine Rehabilitation Center · CNShubun University · JP

Funding

Japanese Society for Electrophysical Agents in Physical Therapy J202307JSPS KAKENHI 21H03852JST SPRING JPMJSP2148
6 · The paper itself

Abstract

purposeExtracellular vesicles (EVs) serve as carriers of intracellular factors with therapeutic effects, including tissue regeneration and attenuation of inflammatory responses. The majority of EVs in vivo are derived from skeletal muscle, which is reported to have anti-inflammatory effects. While high-intensity pulsed ultrasound (US) irradiation has been shown to promote EV secretion from myotubes, the impact of pulse repetition frequency, a US parameter affecting pulse length, on EV release remains unclear. This study aimed to investigate the impact of pulse repetition frequency of US on the release of EVs from myotubes.

methodsC2C12 myoblasts were used in this study. After differentiation into C2C12 myotubes, US was performed for 5 min at an intensity of 3.0 W/cm

resultsUS did not cause a reduction in cell viability across all US groups compared to the control. The concentration of EVs was significantly higher in all US groups compared to the control group. In particular, the highest increase was observed in the 1-Hz group on EV concentration as well as intracellular Ca

conclusionThis study investigated the effect of three different pulse repetition frequencies of US on the release of EVs from cultured myotubes. It is concluded that a low-pulse repetition frequency of 1 Hz is the most effective for enhancing EV release from cultured myotubes with pulsed ultrasound.

Indexed as

Extracellular VesiclesMuscle Fibers, SkeletalUltrasonic WavesAnimalsCalciumCell LineCells, CulturedCell SurvivalMiceCalciumExtracellular vesicles (EVs)Intracellular calciumMyotubesPulsed ultrasoundPulse repetition frequency

Identifiers

PMID38575766
PMCPMC11272820
OpenAlexW4393951401

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.