Evidence map›Paper›PMID 38569552›Full record

ArticleDevelopmental cell2024

Lineage-tracing hematopoietic stem cell origins in vivo to efficiently make human HLF+ HOXA+ hematopoietic progenitors from pluripotent stem cells.

Jonas L Fowler, Sherry Li Zheng, Alana Nguyen, Angela Chen, Xiaochen Xiong, Timothy Chai, Julie Y Chen, Daiki Karigane, Allison M Banuelos, Kouta Niizuma and 16 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed
12.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 38 citations in OpenAlex.

  1. Article
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  4. Article
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  10. Efficient Generation of Functional TCRαβbioRxiv : the preprint server for biology · 2026
    Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Endothelial Heterogeneity in Pulmonary Hypertension.Arteriosclerosis, thrombosis, and vascular biology · 2026
    Review
  16. Review
  17. Article
  18. Article
  19. Review
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

26 authors at 5 institutions in 3 countries.

Jonas L FowlerInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Sherry Li ZhengInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Alana NguyenInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Angela ChenInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Xiaochen XiongInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Timothy ChaiInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Julie Y ChenInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA.
Daiki KariganeInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Division of Hematology, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
Allison M BanuelosInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
Kouta NiizumaInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Kensuke KayamoriInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Division of Hematology, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
Toshinobu NishimuraInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
M Kyle CromerDepartment of Surgery, University of California, San Francisco, San Francisco, CA 94143, USA.
David Gonzalez-PerezDepartment of Drug Discovery, Moffitt Cancer Center, Tampa, FL 33612, USA.
Charlotte MasonDepartment of Drug Discovery, Moffitt Cancer Center, Tampa, FL 33612, USA.
Daniel Dan LiuInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
Leyla YilmazInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
Lucile MiquerolAix-Marseille Université, CNRS UMR 7288, IBDM, Marseille 13288, France.
Matthew H PorteusInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Pediatrics, Stanford University, Stanford, CA 94305, USA.
Vincent C LucaDepartment of Drug Discovery, Moffitt Cancer Center, Tampa, FL 33612, USA.
Ravindra MajetiInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Division of Hematology, Department of Medicine, Stanford University, Stanford, CA 94305, USA.
Hiromitsu NakauchiInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Genetics, Stanford University, Stanford, CA 94305, USA.
Kristy Red-HorseInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Biology, Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.
Irving L WeissmanInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Pathology, Stanford University, Stanford, CA 94305, USA.
Lay Teng AngInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA. Electronic address: layteng@stanford.edu.
Kyle M LohInstitute for Stem Cell Biology & Regenerative Medicine, Stanford University, Stanford, CA 94305, USA; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA. Electronic address: kyleloh@stanford.edu.
California Institute for Regenerative Medicine · USMoffitt Cancer Center · USCentre National de la Recherche Scientifique · FRHoward Hughes Medical Institute · USUniversity of California, San Francisco · US

Funding

Mechanotransduction and transcriptional regulation during artery developmentR01HL128503 · NHLBI · STANFORD UNIVERSITY · PI Mary Red-Horse · 2015 to 2026
$5.8M
Illuminating Notch receptor-ligand selectivity through structure-guided protein engineering supplementR35GM133482 · NIGMS · H. LEE MOFFITT CANCER CTR & RES INST · PI Vince Luca · 2019 to 2026
$3.3M
Program in Translational and Experimental HematologyT32HL120824 · NHLBI · STANFORD UNIVERSITY · PI MAJETI, RAVINDRA · 2014 to 2023
$2.4M
Developing approaches for universal organ transplantationDP5OD024558 · OD · STANFORD UNIVERSITY · PI LOH, KYLE M · 2017 to 2021
$2.0M
Modulating HSC-niche interactions to understand aging and improve transplantationR01HL147124 · NHLBI · STANFORD UNIVERSITY · PI NAKAUCHI, HIROMITSU · 2018 to 2021
$1.6M
Epigenetic, Transcriptional, and Microenvironmental Determinants of Human HSC Self-RenewalR01HL142637 · NHLBI · STANFORD UNIVERSITY · PI MAJETI, RAVINDRA · 2018 to 2021
$1.6M
Xevo TQ-XS Triple Quadrupole Mass Spectrometer SystemS10OD026962 · OD · STANFORD UNIVERSITY · PI CHIEN, ALLIS SOE-LANG · 2019 to 2019
$527k
NHLBI NIH HHS R01 HL128503NHLBI NIH HHS R01 HL142637NHLBI NIH HHS R01 HL147124NHLBI NIH HHS T32 HL120824NIGMS NIH HHS R35 GM133482NIH HHS DP5 OD024558NIH HHS S10 OD026962
6 · The paper itself

Abstract

The developmental origin of blood-forming hematopoietic stem cells (HSCs) is a longstanding question. Here, our non-invasive genetic lineage tracing in mouse embryos pinpoints that artery endothelial cells generate HSCs. Arteries are transiently competent to generate HSCs for 2.5 days (∼E8.5-E11) but subsequently cease, delimiting a narrow time frame for HSC formation in vivo. Guided by the arterial origins of blood, we efficiently and rapidly differentiate human pluripotent stem cells (hPSCs) into posterior primitive streak, lateral mesoderm, artery endothelium, hemogenic endothelium, and >90% pure hematopoietic progenitors within 10 days. hPSC-derived hematopoietic progenitors generate T, B, NK, erythroid, and myeloid cells in vitro and, critically, express hallmark HSC transcription factors HLF and HOXA5-HOXA10, which were previously challenging to upregulate. We differentiated hPSCs into highly enriched HLF+ HOXA+ hematopoietic progenitors with near-stoichiometric efficiency by blocking formation of unwanted lineages at each differentiation step. hPSC-derived HLF+ HOXA+ hematopoietic progenitors could avail both basic research and cellular therapies.

Indexed as

Cell DifferentiationCell LineageHematopoietic Stem CellsPluripotent Stem CellsAnimalsBasic-Leucine Zipper Transcription FactorsEndothelial CellsHematopoiesisHomeodomain ProteinsHumansMiceTranscription FactorsBasic-Leucine Zipper Transcription FactorsHLF protein, humanHomeodomain ProteinsHoxA proteinTranscription Factorsarterydevelopmental biologyhematopoietic stem cellhuman pluripotent stem cell differentiation

Identifiers

PMID38569552
PMCPMC11072092
OpenAlexW4393411138

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.