Evidence map›Paper›PMID 38568383›Full record

ArticleFish physiology and biochemistry2024

Analysis of morphology, histology characteristics, and circadian clock gene expression of Onychostoma macrolepis at the overwintering period and the breeding period.

Yibin Ding, Jincan Li, Yao Gao, Xiaolin Wang, Yang Wang, Chao Zhu, Qimin Liu, Lijuan Zheng, Meng Qi, Lijun Zhang and 4 more

Abstract read
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Article in Fish physiology and biochemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Expression of gastrointestinal clock genes and associated hormones levels in hibernating and non-hibernating Daurian ground squirrels.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Yibin Ding *College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Jincan Li *College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Yao GaoCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Xiaolin WangCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Yang WangCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Chao ZhuBiology Research Centre of Qin Mountains Wildlife, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Qimin LiuCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Lijuan ZhengCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Meng QiChina Institute of Selenium Industry, Ankang, 725000, Shaanxi, China.
Lijun ZhangChina Institute of Selenium Industry, Ankang, 725000, Shaanxi, China.
Hong JiCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Fangxia YangCollege of Forestry, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China.
Xiaoteng FanCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China. fanxiaoteng@nwafu.edu.cn.
Wuzi DongCollege of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, Yangling, 712100, Shaanxi, China. dongwuzi@nwsuaf.edu.cn.

Funding

Fund of Key Sci-Tech Project of Shaanxi Province NO.2018ZDXM-NY-034Research and Development Program of China Se-enriched Industry Research Institute No. 2021FXZX04-01the Key R&D plan Project of Shaanxi Province S2022-YF-YBNY-0055
6 · The paper itself

Abstract

Fish typically adapt to their environment through evolutionary traits, and this adaptive strategy plays a critical role in promoting species diversity. Onychostoma macrolepis is a rare and endangered wild species that exhibits a life history of overwintering in caves and breeding in mountain streams. We analyzed the morphological characteristics, histological structure, and expression of circadian clock genes in O. macrolepis to elucidate its adaptive strategies to environmental changes in this study. The results showed that the relative values of O. macrolepis eye diameter, body height, and caudal peduncle height enlarged significantly during the breeding period. The outer layer of the heart was dense; the ventricular myocardial wall was thickened; the fat was accumulated in the liver cells; the red and white pulp structures of the spleen, renal tubules, and glomeruli were increased; and the goblet cells of the intestine were decreased in the breeding period. In addition, the spermatogenic cyst contained mature sperm, and the ovaries were filled with eggs at various stages of development. Throughout the overwintering period, the melano-macrophage center is located between the spleen and kidney, and the melano-macrophage center in the cytoplasm has the ability to synthesize melanin, and is arranged in clusters to form cell clusters or white pulp scattered in it. Circadian clock genes were identified in all organs, exhibiting significant differences between the before/after overwintering period and the breeding period. These findings indicate that the environment plays an important role in shaping the behavior of O. macrolepis, helping the animals to build self-defense mechanisms during cyclical habitat changes. Studying the morphological, histological structure and circadian clock gene expression of O. macrolepis during the overwintering and breeding periods is beneficial for understanding its unique hibernation behavior in caves. Additionally, it provides an excellent biological sample for investigating the environmental adaptability of atypical cavefish species.

Indexed as

Adaptation, PhysiologicalCircadian ClocksCyprinidaeAnimalsBreedingFemaleFish ProteinsGene ExpressionKidneyLiverMaleSeasonsSexual Behavior, AnimalSpleenFish ProteinsAdaptive strategyCircadian clockHistological structureMorphological characteristicOnychostoma macrolepis

Identifiers

PMID38568383

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.