Evidence map›Paper›PMID 38562781›Full record

ArticlebioRxiv : the preprint server for biology2024

Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels.

Madeline K Eiken, Charlie J Childs, Lindy K Brastrom, Tristan Frum, Eleanor M Plaster, Orren Shachaf, Suzanne Pfeiffer, Justin E Levine, Konstantinos-Dionysios Alysandratos, Darrell N Kotton and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Madeline K EikenDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, USA.
Charlie J ChildsDepartment of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, USA.
Lindy K BrastromDepartment of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA.
Tristan FrumDepartment of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA.
Eleanor M PlasterDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, USA.
Orren ShachafDepartment of Biomedical Engineering, University of Texas, Austin, TX, USA.
Suzanne PfeifferDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, USA.
Justin E LevineDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, USA.
Konstantinos-Dionysios AlysandratosCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
Darrell N KottonCenter for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA 02118, USA.
Jason R SpenceDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, USA.
Claudia LoebelDepartment of Biomedical Engineering, University of Michigan, Ann Arbor, USA.
University of Michigan · USBoston University · USThe University of Texas at Austin · US

Funding

Derivation of Transplantable Lung Epithelial Progenitors from iPS CellsR01HL095993 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Darrell N. Kotton · 2009 to 2026
$7.5M
NATIONAL BIOREPOSITORY OF LUNG DISEASE-SPECIFIC HUMAN INDUCED PLURIPOTENT STEM CELLS:THE PURPOSE OF THIS RESOURCE IS TO BUILD AND SUPPORT A CRITICAL75N92020C00005 · NHLBI · BOSTON UNIVERSITY MEDICAL CAMPUS · PI N/A, N/A · 2021 to 2024
$2.6M
Functional interrogation of a novel SCGB3A2+/SFTPB+ cell in the human airwayR01HL166139 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Jason Spence · 2023 to 2026
$2.4M
Engineered alveolar organoids to understand ECM signalingR00HL151670 · NHLBI · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI LOEBEL, CLAUDIA · 2021 to 2023
$747k
NHLBI NIH HHS 75N92020C00005NHLBI NIH HHS R00 HL151670NHLBI NIH HHS R01 HL095993NHLBI NIH HHS R01 HL166139
6 · The paper itself

Abstract

Human induced pluripotent stem cell (iPSC) derived alveolar organoids have emerged as a system to model the alveolar epithelium in homeostasis and disease. However, alveolar organoids are typically grown in Matrigel, a mouse-sarcoma derived basement membrane matrix that offers poor control over matrix properties, prompting the development of synthetic hydrogels as a Matrigel alternative. Here, we develop a two-step culture method that involves pre-aggregation of organoids in hydrogel-based microwells followed by embedding in a synthetic hydrogel that supports alveolar organoid growth, while also offering considerable control over organoid and hydrogel properties. We find that the aggregated organoids secrete their own nascent extracellular matrix (ECM) both in the microwells and upon embedding in the synthetic hydrogels. Thus, the synthetic gels described here allow us to de-couple exogenous and nascent ECM in order to interrogate the role of ECM in organoid formation.

Identifiers

PMID38562781
PMCPMC10983987
OpenAlexW4392939240

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.