Evidence map›Paper›PMID 38560254›Full record

ArticleHeliyon2024

Molecular network mechanism in cerebral ischemia-reperfusion rats treated with human urine stem cells.

Lang-Chun Zhang, Na Li, Ji-Lin Chen, Jie Sun, Min Xu, Wen-Qiang Liu, Zhong-Fu Zuo, Lan-Lan Shi, Ting-Hua Wang, Xiang-Yin Luo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.5field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Lang-Chun ZhangDepartment of Neurosurgery, Xiang Ya Hospital of Central South University, Changsha, 410078, China.
Na LiAnimal Canter Department of Anatomy, Kunming Medical University, Kunming, 650500, China.
Ji-Lin ChenAnimal Canter Department of Anatomy, Kunming Medical University, Kunming, 650500, China.
Jie SunAnimal Canter Department of Anatomy, Kunming Medical University, Kunming, 650500, China.
Min XuAnimal Canter Department of Anatomy, Kunming Medical University, Kunming, 650500, China.
Wen-Qiang LiuCollege of Basic Medicine, Jinzhou Medical University, Jinzhou, 121000, China.
Zhong-Fu ZuoDepartment of Anatomy, Jinzhou Medical University, Jinzhou, China.
Lan-Lan ShiDepartment of Neurosurgery, Xiang Ya Hospital of Central South University, Changsha, 410078, China.
Ting-Hua WangDepartment of Neurosurgery, Xiang Ya Hospital of Central South University, Changsha, 410078, China.
Xiang-Yin LuoDepartment of Neurosurgery, Xiang Ya Hospital of Central South University, Changsha, 410078, China.
Kunming Medical University · CNCentral South University · CNJinzhou Medical University · CNXiangya Hospital Central South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To explore the effect of human urine-derived stem cells (husc) in improving the neurological function of rats with cerebral ischemia-reperfusion (CIR), and report new molecular network by bioinformatics, combined with experiment validation. Methods: After CIR model was established, and husc were transplanted into the lateral ventricle of rats，neurological severe score (NSS) andgene network analysis were performed. Firstly, we input the keywords "Cerebral reperfusion" and "human urine stem cells" into Genecard database and merged data with findings from PubMed so as to get their targets genes, and downloaded them to make Venny intersection plot. Then, Gene ontology (GO) analysis, kyoto encyclopedia of genes and genomes (KEGG) pathway analysis and protein-protein interaction (PPI) were performed to construct molecular network of core genes. Lastly, the expressional level of core genes was validated via quantitative real-time polymerase chain reaction (qRT-PCR), and localized by immunofluorescence. Results: Compared with the Sham group, the neurological function of CIR rats was significantly improved after the injection of husc into the lateral ventricle; at 14 days, P = 0.028, which was statistically significant. There were 258 overlapping genes between CIR and husc, and integrated with 252 genes screened from PubMed and CNKI. GO enrichment analysis were mainly involved neutrophil degranulation, neutrophil activation in immune response and platelet positive regulation of degranulation, Hemostasis, blood coagulation, coagulation, etc. KEGG pathway analysis was mainly involved in complement and coagulation cascades, ECM-receptor. Hub genes screened by Cytoscape consist ofCD44, ACTB, FN1, ITGB1, PLG, CASP3, ALB, HSP90AA1, EGF, GAPDH. Lastly, qRT-PCR results showed statistic significance (P < 0.05) in ALB, CD44 and EGF before and after treatment, and EGF immunostaining was localized in neuron of cortex. Conclusion: husc transplantation showed a positive effect in improving neural function of CIR rats, and underlying mechanism is involved in CD44, ALB, and EGF network.

Indexed as

BioinformaticsCerebral ischemia reperfusionHuman urine stem cells

Identifiers

PMID38560254
PMCPMC10979071
OpenAlexW4392768002

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.