Evidence map›Paper›PMID 38559262›Full record

ArticlebioRxiv : the preprint server for biology2025

Cell cycle and Age-Related Modulations of Mouse Chromosome Stiffness.

Ning Liu, Wenan Qiang, Philip Jordan, John F Marko, Huanyu Qiao

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Ning LiuDepartment of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Wenan QiangCenter for Developmental Therapeutics, Northwestern University, Evanston, IL, USA.
Philip JordanBiochemistry and Molecular Biology Departments, Johns Hopkins University, Baltimore, MD, USA.
John F MarkoDepartment of Molecular Biosciences, Northwestern University, Evanston, IL, USA 60208.ORCID 0000-0003-4151-9530
Huanyu QiaoDepartment of Comparative Biosciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.ORCID 0000-0003-0966-8077
Northwestern University · USUniversity of Illinois Urbana-Champaign · USJohns Hopkins University · US

Funding

Research Education CoreU54CA203000 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI YANEZ, BETINA · 2015 to 2024
$12.4M
Center for 3D Structure and Physics of the GenomeUM1HG011536 · NHGRI · UNIV OF MASSACHUSETTS MED SCH WORCESTER · PI DEKKER, JOB, MIRNY, LEONID A · 2020 to 2024
$11.8M
Spatio-Temporal Organization of Chromatin and Information Transfer in CancerU54CA193419 · NCI · NORTHWESTERN UNIVERSITY AT CHICAGO · PI O'HALLORAN, THOMAS V · 2015 to 2020
$10.4M
Research Training Program in Toxicology and Environmental HealthT32ES007326 · NIEHS · UNIVERSITY OF ILLINOIS URBANA-CHAMPAIGN · PI Jodi A. Flaws · 2000 to 2026
$8.7M
Regulation of microtubule organizing centers during mammalian gametogenesisR01GM117155 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Philip W Jordan · 2016 to 2026
$4.3M
Activities of nucleoprotein complexes visualized in single-molecule experimentsR01GM105847 · NIGMS · NORTHWESTERN UNIVERSITY · PI MARKO, JOHN F · 2013 to 2025
$2.5M
Micromechanical basis of meiotic chromosome condensation and architectureR01GM135549 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI QIAO, HUANYU · 2020 to 2024
$2.2M
Meiotic checkpoint pathways and gametocyte quality controlR00HD082375 · NICHD · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI QIAO, HUANYU · 2017 to 2019
$674k
Meiotic checkpoint pathways and gametocyte quality controlK99HD082375 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI QIAO, HUANYU · 2015 to 2016
$178k
NCI NIH HHS U54 CA193419NCI NIH HHS U54 CA203000NHGRI NIH HHS UM1 HG011536NICHD NIH HHS K99 HD082375NICHD NIH HHS R00 HD082375NIEHS NIH HHS T32 ES007326NIGMS NIH HHS R01 GM105847NIGMS NIH HHS R01 GM117155NIGMS NIH HHS R01 GM135549
6 · The paper itself

Abstract

Chromosome structure is complex, and many aspects of chromosome organization are still not understood. Measuring the stiffness of chromosomes offers valuable insight into their structural properties. In this study, we analyzed the stiffness of chromosomes from metaphase I (MI) and metaphase II (MII) oocytes. Our results revealed a ten-fold increase in stiffness (Young's modulus) of MI chromosomes compared to somatic chromosomes. Furthermore, the stiffness of MII chromosomes was found to be lower than that of MI chromosomes. We examined the role of meiosis-specific cohesin complexes in regulating chromosome stiffness. Surprisingly, the stiffness of chromosomes from three meiosis-specific cohesin mutants did not significantly differ from that of wild-type chromosomes, indicating that these cohesins may not be primary determinants of chromosome stiffness. Additionally, our findings revealed an age-related increase of chromosome stiffness for MI oocytes. Since aging is associated with elevated levels of DNA damage, we investigated the impact of etoposide-induced DNA damage on chromosome stiffness and found that it led to a reduction in stiffness in MI oocytes. Overall, our study underscores the dynamic and cyclical nature of chromosome stiffness, modulated by both the cell cycle, and by age-related factors.

Indexed as

agecell cyclechromosome stiffnesscohesin proteinmeiosisoocytespermatocyte

Identifiers

PMID38559262
PMCPMC10979861
OpenAlexW4392714560

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.