Evidence map›Paper›PMID 38559123›Full record

ArticlebioRxiv : the preprint server for biology2025

Single-Cell Atlas Uncovers Layer 4 Heterogeneity and Functional Gradients in Rhesus Macaque Visual Cortex.

Dorothee M Günther, Mykhailo Y Batiuk, Romain De Oliveira, Viktor Petukhov, Thomas Wunderle, Christian J Buchholz, Pascal Fries, Konstantin Khodosevich

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 4 countries.

Dorothee M GüntherErnst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, 60528 Frankfurt, Germany.
Mykhailo Y BatiukBiotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Romain De OliveiraBiotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Viktor PetukhovBiotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Thomas WunderleErnst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, 60528 Frankfurt, Germany.
Christian J BuchholzMolecular Biotechnology and Gene Therapy, Paul-Ehrlich-Institut, 63225 Langen, Germany.
Pascal FriesErnst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, 60528 Frankfurt, Germany.
Konstantin KhodosevichBiotech Research and Innovation Centre (BRIC), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
University of Copenhagen · DKRadboud University Nijmegen · NLErnst Strüngmann Institute for Neuroscience · DEPaul Ehrlich Institut · DE

Funding

Mapping the Human Connectome: Structure, Function, and HeritabilityU54MH091657 · NIMH · WASHINGTON UNIVERSITY · PI UGURBIL, KAMIL, VAN ESSEN, DAVID C · 2010 to 2014
$34.7M
NIMH NIH HHS U54 MH091657
6 · The paper itself

Abstract

Non-human primates like rhesus macaques are pivotal models for decoding human visual cortex physiology and disorders. We introduce BrainSPACE, an innovative pipeline for rapid and precise brain tissue banking, to sample visual cortex areas: V1, V2, V4, MT, and TEO. Applying snRNA-seq to V1 and V4 (95,071 nuclei), we uncovered conserved GABAergic neuron profiles but stark area-specific diversity in principal neurons, featuring seven unique layer 4 subtypes in V1 and one in V4. Complementary smFISH validated transcriptional gradients across these areas, aligning with ventral and dorsal stream hierarchies. Gene ontology analyses highlighted plasticity-related pathways in unique layer 4 subtypes, with genes like

Identifiers

PMID38559123
PMCPMC10979896
OpenAlexW4392747658

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.