Evidence map›Paper›PMID 38558968›Full record

ArticlebioRxiv : the preprint server for biology2025

CROPseq-multi: a universal solution for multiplexed perturbation in high-content pooled CRISPR screens.

Russell T Walton, Yue Qin, Bryce Kirby, J Owen Andrews, Mikko Taipale, Byunguk Kang, Paul C Blainey

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Russell T WaltonBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID 0000-0002-0546-474X
Yue QinBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID 0000-0002-0496-7134
Bryce KirbyBroad Institute of MIT and Harvard, Cambridge, MA, USA.
J Owen AndrewsBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Mikko TaipaleDonnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, ON, Canada.ORCID 0000-0003-3811-1761
Byunguk KangBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID 0009-0004-5540-7745
Paul C BlaineyBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID 0000-0002-4889-8783

Funding

Targeting Dysregulated RNA Splicing in Neurodegenerative DiseasesRM1NS133601 · NINDS · MASSACHUSETTS GENERAL HOSPITAL · PI Paul Clark Blainey, Clotilde Lagier-Tourenne · 2023 to 2026
$7.4M
Epigenetic Pathology and Therapy in Huntington's DiseaseR01NS089076 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI FRAENKEL, ERNEST, HOUSMAN, DAVID · 2015 to 2024
$4.9M
Unraveling the Genetic Programs Engaged in ASD Neurons Through Coupled Transcriptomic and Phenotypic ReadoutsR01MH128366 · NIMH · BROAD INSTITUTE, INC. · PI Samouil Farhi, Ralda Nehme · 2022 to 2026
$3.7M
Profiling and perturbing the inheritance of drug-induced metabolic states in cancer with Inheritance-SeqR61CA278536 · NCI · BROAD INSTITUTE, INC. · PI Paul Clark Blainey · 2024 to 2026
$621k
A visible machine learning system to discover targeted treatment solutions in cancerK00CA264422 · NCI · BROAD INSTITUTE, INC. · PI Yue Qin · 2023 to 2026
$395k
NCI NIH HHS K00 CA264422NCI NIH HHS R61 CA278536NIMH NIH HHS R01 MH128366NINDS NIH HHS R01 NS089076NINDS NIH HHS RM1 NS133601
6 · The paper itself

Abstract

Forward genetic screens seek to dissect complex biological systems by systematically perturbing genetic elements and observing the resulting phenotypes. While standard screening methodologies introduce individual perturbations, multiplexing perturbations improves the performance of single-target screens and enables combinatorial screens for the study of genetic interactions. Current tools for multiplexing perturbations are limited by technical challenges and do not offer compatibility across diverse screening methodologies, including enrichment, single-cell sequencing, and optical pooled screens. Here, we report the development of CROPseq-multi (CSM), a CROPseq

Indexed as

CRISPR Cas9CRISPRiCRISPR knockoutgenetic interactionshigh-content screeningmultiplexed perturbationoptical pooled screensPerturb-seq

Identifiers

PMID38558968
PMCPMC10979941

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.