Evidence map›Paper›PMID 38558151›Full record

ArticleApplied microbiology and biotechnology2024

Mutation breeding of high-stress resistant strains for succinic acid production from corn straw.

Jing Wu, Yilian Li, Jinbao Yin, Chen Wang, Xuejin Qi, Yujie Zhou, Hongjuan Liu, Pengfei Wu, Jianan Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jing WuShanxi Key Laboratory of Chemical Product Engineering, College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan, 030024, China.ORCID http://orcid.org/0000-0003-2793-0032
Yilian LiShanxi Key Laboratory of Chemical Product Engineering, College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan, 030024, China.
Jinbao YinShanxi Key Laboratory of Chemical Product Engineering, College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan, 030024, China.
Chen WangShanxi Key Laboratory of Chemical Product Engineering, College of Chemical Engineering and Technology, Taiyuan University of Technology, Taiyuan, 030024, China.
Xuejin QiInstitute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Yujie ZhouInstitute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Hongjuan LiuInstitute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
Pengfei WuCollege of Life Science and Technology, Yangtze Normal University, Fuling Chongqing, 408100, China. wpf5101@163.com.
Jianan ZhangInstitute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China. zhangja@tsinghua.edu.cn.

Funding

Basic Research Program (Free Exploration) Project of Shanxi Province RD2100005716National Key R&D Program of China No.2016YEE0131300
6 · The paper itself

Abstract

The production of succinic acid from corn stover is a promising and sustainable route; however, during the pretreatment stage, byproducts such as organic acids, furan-based compounds, and phenolic compounds generated from corn stover inhibit the microbial fermentation process. Selecting strains that are resistant to stress and utilizing nondetoxified corn stover hydrolysate as a feedstock for succinic acid production could be effective. In this study, A. succinogenes CICC11014 was selected as the original strain, and the stress-resistant strain A. succinogenes M4 was obtained by atmospheric and room temperature plasma (ARTP) mutagenesis and further screening. Compared to the original strain, A. succinogenes M4 exhibited a twofold increase in stress resistance and a 113% increase in succinic acid production when hydrolysate was used as the substrate. By conducting whole-genome resequencing of A. succinogenes M4 and comparing it with the original strain, four nonsynonymous gene mutations and two upstream regions with base losses were identified. KEY POINTS: • A high-stress-resistant strain A. succinogenes M4 was obtained by ARTP mutation •  The production of succinic acid increased by 113% • The mutated genes of A. succinogenes M4 were detected and analyzed.

Indexed as

ActinobacillusZea maysFermentationMutationPlant BreedingSuccinic AcidSuccinic AcidActinobacillus succinogenesAtmospheric room temperature plasmaCorn strawFermentationSuccinic acid

Identifiers

PMID38558151
PMCPMC10984890

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.