Evidence map›Paper›PMID 38556880›Full record

ArticleBMC genomics2024

Transcriptomic changes in the microsporidia proliferation and host responses in congenitally infected embryos and larvae.

Zigang Shen, Zhuojun Ke, Qiong Yang, Samson Teweldeberhan Ghebremichael, Tangxin Li, Tian Li, Jie Chen, Xianzhi Meng, Heng Xiang, Chunfeng Li and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.0field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 1 country.

Zigang ShenCollege of Sericulture, Textile and Biomass Sciences, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Zhuojun KeState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Qiong YangSericulture and Agri-food Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, China.
Samson Teweldeberhan GhebremichaelState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Tangxin LiState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Tian LiState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Jie ChenState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Xianzhi MengState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Heng XiangCollege of Animal Science and Technology, Southwest University, Chongqing, China.
Chunfeng LiState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Zeyang ZhouState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China.
Guoqing PanState Key Laboratory of Resource Insects, Southwest University, Tiansheng Street, Chongqing, 400716, China. gqpan@swu.edu.cn.
Ping ChenCollege of Sericulture, Textile and Biomass Sciences, Southwest University, Tiansheng Street, Chongqing, 400716, China. chenping1918@swu.edu.cn.
Southwest University · CNGuangdong Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Congenital infection caused by vertical transmission of microsporidia N. bombycis can result in severe economic losses in the silkworm-rearing industry. Whole-transcriptome analyses have revealed non-coding RNAs and their regulatory networks in N. bombycis infected embryos and larvae. However, transcriptomic changes in the microsporidia proliferation and host responses in congenitally infected embryos and larvae remains unclear. Here, we simultaneously compared the transcriptomes of N. bombycis and its host B. mori embryos of 5-day and larvae of 1-, 5- and 10-day during congenital infection. For the transcriptome of N. bombycis, a comparison of parasite expression patterns between congenital-infected embryos and larva showed most genes related to parasite central carbon metabolism were down-regulated in larvae during infection, whereas the majority of genes involved in parasite proliferation and growth were up-regulated. Interestingly, a large number of distinct or shared differentially expressed genes (DEGs) were revealed by the Venn diagram and heat map, many of them were connected to infection related factors such as Ricin B lectin, spore wall protein, polar tube protein, and polysaccharide deacetylase. For the transcriptome of B. mori infected with N. bombycis, beyond numerous DEGs related to DNA replication and repair, mRNA surveillance pathway, RNA transport, protein biosynthesis, and proteolysis, with the progression of infection, a large number of DEGs related to immune and infection pathways, including phagocytosis, apoptosis, TNF, Toll-like receptor, NF-kappa B, Fc epsilon RI, and some diseases, were successively identified. In contrast, most genes associated with the insulin signaling pathway, 2-oxacarboxylic acid metabolism, amino acid biosynthesis, and lipid metabolisms were up-regulated in larvae compared to those in embryos. Furthermore, dozens of distinct and three shared DEGs that were involved in the epigenetic regulations, such as polycomb, histone-lysine-specific demethylases, and histone-lysine-N-methyltransferases, were identified via the Venn diagram and heat maps. Notably, many DEGs of host and parasite associated with lipid-related metabolisms were verified by RT-qPCR. Taken together, simultaneous transcriptomic analyses of both host and parasite genes lead to a better understanding of changes in the microsporidia proliferation and host responses in embryos and larvae in N. bombycis congenital infection.

Indexed as

BombyxNosemaAnimalsCell ProliferationGene Expression ProfilingHistonesLarvaLipidsLysineTranscriptomeHistonesLipidsLysineB. moriCongenital infectionEpigenetic regulationImmune responseMetabolismN. BombycisProliferation

Identifiers

PMID38556880
PMCPMC10983672
OpenAlexW4393391086

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.