Evidence map›Paper›PMID 38554181›Full record

ArticlePlanta2024

OsmiR5519 regulates grain size and weight and down-regulates sucrose synthase gene RSUS2 in rice (Oryza sativa L.).

Shengyuan Guo, Yajuan Li, Yan Wang, Yangwen Xu, Yuting Li, Ping Wu, Jinwen Wu, Lan Wang, Xiangdong Liu, Zhixiong Chen

Abstract read
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Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
4.2field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 1 institution in 1 country.

Shengyuan Guo *Department of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Yajuan Li *Experimental Basis and Practical Training Center, South China Agricultural University, Guangzhou, 510642, China.
Yan WangDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Yangwen XuDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Yuting LiDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Ping WuDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou, 510642, China.
Jinwen WuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
Lan WangState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
Xiangdong LiuState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China. xdliu@scau.edu.cn.
Zhixiong ChenState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China. chenzx@scau.edu.cn.
South China Agricultural University · CN

Funding

Guangdong Base Bank for Lingnan Rice Germplasm Resources Project 2023Natural Science Foundation of Guangdong Province 2019A1515012083the National Natural Science Foundation of China U22A20468
6 · The paper itself

Abstract

MAIN

conclusionThe up-regulation of OsmiR5519 results in the decrease of grain size, weight and seed setting rate. OsmiR5519 plays important roles in the process of grain filling and down-regulates sucrose synthase gene RSUS2. MicroRNAs (miRNAs) are one class of small non-coding RNAs that act as crucial regulators of plant growth and development. In rice, the conserved miRNAs were revealed to regulate the yield components, but the function of rice-specific miRNAs has been rarely studied. The rice-specific OsmiR5519 was found to be abundantly expressed during reproductive development, but its biological roles remain unknown. In this study, the function of rice-specific OsmiR5519 was characterized with the miR5519-overexpressing line (miR5519-OE) and miR5519-silenced line (STTM5519). At seedling stage, the content of sucrose, glucose and fructose was obviously lower in the leaves of miR5519-OE lines than those of wild-type (WT) line. The grain size and weight were decreased significantly in miR5519-OE lines, compared to those of WT rice. The cell width of hull in miR5519-OE was smaller than that in WT. The seed setting rate was notably reduced in miR5519-OE lines, but not in STTM5519 lines. Cytological observation demonstrated that the inadequate grain filling was the main reason for the decline of seed setting rate in miR5519-OE lines. The percentage of the defects of grain amounted to 40% in miR5519-OE lines, which almost equaled to the decreased value of seed setting rate. Furthermore, the sucrose synthase gene RSUS2 was identified as a target of OsmiR5519 via RNA ligase-mediated 3'-amplification of cDNA ends (3'-RLM-RACE), dual luciferase assays and transient expression assays. In summary, our results suggest that OsmiR5519 regulates grain size and weight and down-regulates RSUS2 in rice.

Indexed as

GlucosyltransferasesMicroRNAsOryzaEdible GrainGene Expression Regulation, PlantPlant ProteinsSeedsGlucosyltransferasesMicroRNAsPlant Proteinssucrose synthaseGrain fillingGrain sizeGrain weightRice-specific miRNASeed setting rate

Identifiers

PMID38554181
OpenAlexW4393345586

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.