ArticleNature communications2024
Projective light-sheet microscopy with flexible parameter selection.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed.
- Rapid and gentle volumetric imaging of host-pathogen interactions in salmon skin cells using projective oblique plane microscopy.Scientific reports · 2025Article
- Extended-depth of field random illumination microscopy, EDF-RIM, provides super-resolved projective imaging.Light, science & applications · 2024Article
- Mechanically sheared axially swept light-sheet microscopy.Biomedical optics express · 2024Article
- Adaptive optics in an oblique plane microscope.Biomedical optics express · 2024Article
- Light sheet illumination in single-molecule localization microscopy for imaging of cellular architectures and molecular dynamics.Npj imaging · 2024Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Projection imaging accelerates volumetric interrogation in fluorescence microscopy, but for multi-cellular samples, the resulting images may lack contrast, as many structures and haze are summed up. Here, we demonstrate rapid projective light-sheet imaging with parameter selection (props) of imaging depth, position and viewing angle. This allows us to selectively image different sub-volumes of a sample, rapidly switch between them and exclude background fluorescence. Here we demonstrate the power of props by functional imaging within distinct regions of the zebrafish brain, monitoring calcium firing inside muscle cells of moving Drosophila larvae, super-resolution imaging of selected cell layers, and by optically unwrapping the curved surface of a Drosophila embryo. We anticipate that props will accelerate volumetric interrogation, ranging from subcellular to mesoscopic scales.
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Registered trials
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