Evidence map›Paper›PMID 38549185›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2024

Chronic Opioid Treatment Arrests Neurodevelopment and Alters Synaptic Activity in Human Midbrain Organoids.

Hye Sung Kim, Yang Xiao, Xuejing Chen, Siyu He, Jongwon Im, Moshe J Willner, Michael O Finlayson, Cong Xu, Huixiang Zhu, Se Joon Choi and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
15.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it, 14 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Morphine-inducedFrontiers in neuroscience · 2026
    Article
  4. Review
  5. Article
  6. Article
  7. Review
  8. Review
  9. Article
  10. Article
  11. Review
  12. Article
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 2 institutions in 3 countries.

Hye Sung KimDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.ORCID 0000-0002-2182-301X
Yang XiaoDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.ORCID 0000-0001-7878-4923
Xuejing ChenDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
Siyu HeDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
Jongwon ImDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
Moshe J WillnerDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
Michael O FinlaysonSingle Cell Analysis Core, JP Sulzberger Columbia Genome Center, Columbia University Irving Medical Center, New York, NY, 10032, USA.
Cong XuDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.
Huixiang ZhuDepartment of Psychiatry, Columbia University Medical Center, New York, NY, 10032, USA.
Se Joon ChoiDepartment of Psychiatry, Columbia University Medical Center, New York, NY, 10032, USA.
Eugene V MosharovDepartment of Psychiatry, Columbia University Medical Center, New York, NY, 10032, USA.
Hae-Won KimInstitute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 31116, Republic of Korea.
Bin XuDepartment of Psychiatry, Columbia University Medical Center, New York, NY, 10032, USA.
Kam W LeongDepartment of Biomedical Engineering, Columbia University, New York, NY, 10027, USA.ORCID 0000-0002-8133-4955
Columbia University · USColumbia University Irving Medical Center · US

Funding

Integrated Microphysiological System of Cerebral Organoid and Blood Vessel for Disease Modeling and Neuropsychiatric Drug screeningUH3TR002151 · NCATS · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI LEONG, KAM W · 2020 to 2022
$3.6M
Dankook University Global Research ProgramDankook University Priority Institute Support Program in 2021Foundation for the National Institutes of Health UH3TR002151National Research Foundation of Korea 2021R1A5A2022318National Research Foundation of Korea 2022R1F1A1065132NCATS NIH HHS UH3 TR002151
6 · The paper itself

Abstract

Understanding the impact of long-term opioid exposure on the embryonic brain is critical due to the surging number of pregnant mothers with opioid dependency. However, this has been limited by human brain inaccessibility and cross-species differences in animal models. Here, a human midbrain model is established that uses hiPSC-derived midbrain organoids to assess cell-type-specific responses to acute and chronic fentanyl treatment and fentanyl withdrawal. Single-cell mRNA sequencing of 25,510 cells from organoids in different treatment groups reveals that chronic fentanyl treatment arrests neuronal subtype specification during early midbrain development and alters synaptic activity and neuron projection. In contrast, acute fentanyl treatment increases dopamine release but does not significantly alter gene expression related to cell lineage development. These results provide the first examination of the effects of opioid exposure on human midbrain development at the single-cell level.

Indexed as

Analgesics, OpioidMesencephalonOrganoidsFentanylHumansNeurogenesisAnalgesics, OpioidFentanylbrain organoidfentanylneurodevelopmentopioidsingle‐cell RNA sequencing

Identifiers

PMID38549185
PMCPMC11151039
OpenAlexW4393314561

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.