Evidence map›Paper›PMID 38547833›Full record

ArticleBiomaterials2024

FAK, vinculin, and talin control mechanosensitive YAP nuclear localization.

Elijah N Holland, Marc A Fernández-Yagüe, Dennis W Zhou, Eric B O'Neill, Ayanna U Woodfolk, Ana Mora-Boza, Jianping Fu, David D Schlaepfer, Andrés J García

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.

0numbers the graph read from it
0cells of the map it votes in
39citing papers in PubMed
13.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

39 citing papers in PubMed, 41 citations in OpenAlex.

  1. Review
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  5. Article
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  8. Review
  9. Mechanical cues regulateMaterials today. Bio · 2026
    Article
  10. Article
  11. Review
  12. Review
  13. Article
  14. Mechanobiology of the Hippo-YAP Signaling Network.Cold Spring Harbor perspectives in biology · 2026
    Article
  15. Review
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Elijah N HollandPetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA; School of Chemical and Biomolecular Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Marc A Fernández-YagüePetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA; Department of Chemistry, Queen Mary University of London, London, UK.
Dennis W ZhouPetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA; Coulter Department of Biomedical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Eric B O'NeillPetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Ayanna U WoodfolkMathematics Department, Spelman College, Atlanta, GA, USA; Bioengineering Department, North Carolina A&T State University, Greensboro, NC, USA.
Ana Mora-BozaPetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Jianping FuDepartment of Mechanical Engineering, Department of Biomedical Engineering, Department of Cell & Developmental Biology, University of Michigan, Ann Arbor, MI, USA.
David D SchlaepferMoores Cancer Center, Department of Obstetrics, Gynecology, and Reproductive Sciences, University of California, San Diego, La Jolla, CA, USA.
Andrés J GarcíaPetit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA. Electronic address: andres.garcia@me.gatech.edu.
Georgia Institute of Technology · USSpelman College · USUniversity of California San Diego · USUniversity of Michigan · US

Funding

Dissecting FAK-regulated oncogenic signaling programs in ovarian cancerR01CA247562 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SCHLAEPFER, DAVID D, STUPACK, DWAYNE G. · 2020 to 2024
$2.3M
Reprogramming the Tumor Microenvironment in Ovarian CancerR01CA254342 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SCHLAEPFER, DAVID D · 2020 to 2024
$2.1M
Mechanosensing at Focal AdhesionsR01EB024322 · NIBIB · GEORGIA INSTITUTE OF TECHNOLOGY · PI GARCIA, ANDRES J · 2018 to 2022
$1.3M
NCI NIH HHS R01 CA247562NCI NIH HHS R01 CA254342NIBIB NIH HHS R01 EB024322
6 · The paper itself

Abstract

Focal adhesions (FAs) are nanoscale complexes containing clustered integrin receptors and intracellular structural and signaling proteins that function as principal sites of mechanotransduction in part via promoting the nuclear translocation and activation of the transcriptional coactivator yes-associated protein (YAP). Knockdown of FA proteins such as focal adhesion kinase (FAK), talin, and vinculin can prevent YAP nuclear localization. However, the mechanism(s) of action remain poorly understood. Herein, we investigated the role of different functional domains in vinculin, talin, and FAK in regulating YAP nuclear localization. Using genetic or pharmacological inhibition of fibroblasts and human mesenchymal stem cells (hMSCs) adhering to deformable substrates, we find that disruption of vinculin-talin binding versus talin-FAK binding reduces YAP nuclear localization and transcriptional activity via different mechanisms. Disruption of vinculin-talin binding or knockdown of talin-1 reduces nuclear size, traction forces, and YAP nuclear localization. In contrast, disruption of the talin binding site on FAK or elimination of FAK catalytic activity did not alter nuclear size yet still prevented YAP nuclear localization and activity. These data support both nuclear tension-dependent and independent models for matrix stiffness-regulated YAP nuclear localization. Our results highlight the importance of vinculin-talin-FAK interactions at FAs of adherent cells, controlling YAP nuclear localization and activity.

Indexed as

Cell NucleusMechanotransduction, CellularTalinVinculinYAP-Signaling ProteinsAdaptor Proteins, Signal TransducingAnimalsFibroblastsFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesFocal AdhesionsHumansMesenchymal Stem CellsMiceProtein BindingTranscription FactorsAdaptor Proteins, Signal TransducingFocal Adhesion Kinase 1Focal Adhesion Protein-Tyrosine KinasesPTK2 protein, humanTalinTranscription FactorsVinculinYAP1 protein, humanYAP-Signaling ProteinsFocal adhesionMechanotransductionMicropillar arrayRigidity sensing

Identifiers

PMID38547833
PMCPMC11065566
OpenAlexW4392979678

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.