ArticlePloS one2024
Saliva sampling method influences oral microbiome composition and taxa distribution associated with oral diseases.
Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 12 citations in OpenAlex.
- Oral microbiome diversity and composition before and after chemotherapy treatment in pediatric oncology patients.BMC oral health · 2025Trial
- Salivary innate immune peptides are associated with dental caries experience in young adults.Clinical oral investigations · 2026Article
- Cranberry Polyphenol Extract (CPE) Oral Rinse Improves Salivary Microbiome in 6-n-Propylthiouracil (PROP) Non-Tasters and Palatability of Aronia Juice.International journal of molecular sciences · 2026Article
- Methodological considerations in Mendelian randomization analyses of oral microbiota and cardiovascular disease.Journal of geriatric cardiology : JGC · 2026Article
- Mapping the oral microbiome in children with oral diseases using next-generation sequencing: a systematic review.Journal of oral microbiology · 2026Review
- Anaerobic stress index to predict microbial ecological shifts in clear aligners.Journal of oral microbiology · 2026Article
- Proteomic analysis of saliva reveals changes in proteomic profiles during colorectal cancer in an Iranian cohort.Frontiers in oncology · 2026Article
- Oral dysbiosis: methodological evolution, the mobile resistome and the future of machine learning in dentistry.Journal of oral microbiology · 2026Review
- Impact of shared spaces and daily routines on shared microbiome.Scientific reports · 2025Article
- Review
- Advances in Sinonasal Microbiome Analysis.Ear, nose, & throat journal · 2025Article
- Saliva as a Diagnostic Tool for Systemic Diseases-A Narrative Review.Medicina (Kaunas, Lithuania) · 2025Review
- Impact of increasedJournal of oral microbiology · 2025Article
- The link between inflammatory/ SCFA profiles and oral/gut microbiome: an observational study in patients with ST-segment elevation myocardial infarction.Current research in microbial sciences · 2025Article
- Development and Evaluation of the Biological Activities of a Plain Mucoadhesive Hydrogel as a Potential Vehicle for Oral Mucosal Drug Delivery.Gels (Basel, Switzerland) · 2024Article
- Higher Prevalence of the Periodontal PathogenPathogens (Basel, Switzerland) · 2024Article
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Saliva is a readily accessible and inexpensive biological specimen that enables investigation of the oral microbiome, which can serve as a biomarker of oral and systemic health. There are two routine approaches to collect saliva, stimulated and unstimulated; however, there is no consensus on how sampling method influences oral microbiome metrics. In this study, we analyzed paired saliva samples (unstimulated and stimulated) from 88 individuals, aged 7-18 years. Using 16S rRNA gene sequencing, we investigated the differences in bacterial microbiome composition between sample types and determined how sampling method affects the distribution of taxa associated with untreated dental caries and gingivitis. Our analyses indicated significant differences in microbiome composition between the sample types. Both sampling methods were able to detect significant differences in microbiome composition between healthy subjects and subjects with untreated caries. However, only stimulated saliva revealed a significant association between microbiome diversity and composition in individuals with diagnosed gingivitis. Furthermore, taxa previously associated with dental caries and gingivitis were preferentially enriched in individuals with each respective disease only in stimulated saliva. Our study suggests that stimulated saliva provides a more nuanced readout of microbiome composition and taxa distribution associated with untreated dental caries and gingivitis compared to unstimulated saliva.
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Registered trials
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