A simple and rapid HPLC-UV method for the determination of valproic acid in human plasma using microwave-assisted derivatization with phenylhydrazine hydrochloride.

Sirikanya Kaewpradit, Gorawit Yusakul, Pornchai Rojsitthisak, Chutima Jantarat

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Article in Heliyon, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

Sirikanya KaewpraditSchool of Pharmacy, Walailak University, Thasala, Nakhon Si Thammarat, 80160, Thailand.

Gorawit YusakulSchool of Pharmacy, Walailak University, Thasala, Nakhon Si Thammarat, 80160, Thailand.

Pornchai RojsitthisakDepartment of Food and Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.

Chutima JantaratSchool of Pharmacy, Walailak University, Thasala, Nakhon Si Thammarat, 80160, Thailand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study presents an efficient high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method for monitoring valproic acid (VPA) level in human plasma. This method is distinguished by its simplicity, cost-effectiveness, and rapid execution, addressing the limitations associated with other advanced analytical techniques like liquid chromatography-mass spectrometry (LC-MS), gas chromatography-mass spectrometry (GC-MS), and immunoassays, which are generally complex and costly for routine application. A challenge in analyzing VPA is its non-linear protein binding profile and the absence of a chromophore in its structure, making direct detection difficult. To overcome this, the study developed an efficient HPLC-UV for VPA determination in human plasma, utilizing a simplified and rapid microwave-assisted derivatization process. Due to the lack of a chromophore in VPA structure, this work developed a microwave-assisted derivatization of VPA using phenylhydrazine hydrochloride (PH HCl). The process optimization was achieved at 450 W for 50 s, facilitating effective HPLC-UV detection. The derivatized product was characterized using

Indexed as

Human plasmaHydrazoneMicrowave assisted derivatizationPhenylhydrazine hydrochloridePrecolumn derivatizationValproic acid

Identifiers

PMID38545211

DOI10.1016/j.heliyon.2024.e27875

PMCPMC10965533

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