Evidence map›Paper›PMID 38542970›Full record

ArticleMolecules (Basel, Switzerland)2024

Purification and Biochemical Characterization of Polyphenol Oxidase Extracted from Wheat Bran (

Kun Yu, Wei He, Xiaoli Ma, Qi Zhang, Chunxu Chen, Peiyan Li, Di Wu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecules (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
3.8field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 17 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Kun YuSchool of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China.
Wei HeSchool of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China.
Xiaoli MaSchool of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China.
Qi ZhangSchool of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China.
Chunxu ChenSchool of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China.ORCID 0000-0003-4716-6553
Peiyan LiSchool of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China.
Di WuSchool of Food Engineering, Anhui Science and Technology University, Chuzhou 233100, China.
Anhui University of Science and Technology · CN

Funding

Anhui Provincial Central Government Guiding Local Science and Technology Development Project 202107d06020021key projects of Anhui Provincial Department of Education 2022AH051647talent foundation of Anhui Science and Technology University SPYJ202102
6 · The paper itself

Abstract

Currently, little is known about the characteristics of polyphenol oxidase from wheat bran, which is closely linked to the browning of wheat product. The wheat PPO was purified by ammonium sulfate precipitation, DEAE-Sepharose ion-exchange column, and Superdex G-75 chromatography column. Purified wheat PPO activity was 11.05-fold higher, its specific activity was 1365.12 U/mg, and its yield was 8.46%. SDS-PAGE showed that the molecular weight of wheat PPO was approximately 21 kDa. Its optimal pH and temperature were 6.5 and 35 °C for catechol as substrate, respectively. Twelve phenolic substrates from wheat and green tea were used for analyzing the substrate specificity. Wheat PPO showed the highest affinity to catechol due to its maximum V

Indexed as

Catechol OxidaseDietary FiberCatecholsHydrogen-Ion ConcentrationKineticsPlant ProteinsSubstrate SpecificityTeacatecholCatechol OxidaseCatecholsDietary FiberPlant ProteinsTeabiochemical characterizationpurificationwheat polyphenol oxidase

Identifiers

PMID38542970
PMCPMC10976219
OpenAlexW4392921393

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.