Evidence map›Paper›PMID 38542482›Full record

ArticleInternational journal of molecular sciences2024

Fine-Tuning of DADA2 Parameters for Multiregional Metabarcoding Analysis of 16S rRNA Genes from Activated Sludge and Comparison of Taxonomy Classification Power and Taxonomy Databases.

Wiktor Babis, Jan P Jastrzebski, Slawomir Ciesielski

Abstract read
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Special Issue "Current Research on Omics of Microorganisms".International journal of molecular sciences · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Wiktor BabisDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.
Jan P JastrzebskiDepartment of Plant Physiology, Genetics and Biotechnology, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland.ORCID 0000-0001-8699-7742
Slawomir CiesielskiDepartment of Environmental Biotechnology, University of Warmia and Mazury in Olsztyn, 11-709 Olsztyn, Poland.ORCID 0000-0002-9084-9782

Funding

The National Centre for Research and Development, Program "Applied research" implemented under the Norwegian Financial Mechanism 2014-2021/POLNOR 2019 NOR/POLNOR/SIREN/0069/2019-00
6 · The paper itself

Abstract

Taxonomic classification using metabarcoding is a commonly used method in microbiological studies of environmental samples and during monitoring of biotechnological processes. However, it is difficult to compare results from different laboratories, due to the variety of bioinformatics tools that have been developed and used for data analysis. This problem is compounded by different choices regarding which variable region of the

Indexed as

BacteriaSewageGenes, rRNAHigh-Throughput Nucleotide SequencingRNA, Ribosomal, 16SSequence Analysis, DNARNA, Ribosomal, 16SSewage16S rRNA geneactivated sludgebiodiversityDADA2metabarcodingOTUs

Identifiers

PMID38542482
PMCPMC10971298

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.