ArticleInternational journal of molecular sciences2024
Development of Genome-Wide Intron Length Polymorphism (ILP) Markers in Tea Plant (
Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Genetic diversity and population structure of wild and cultivated Camellia tetracocca Chang assessed by ILP markers: insights for conservation of an endangered tea species.BMC plant biology · 2026Article
- CamK-DB: A k-mer MinHash fingerprint database for reference-free genotyping of Camellia accessions.GigaScience · 2026Article
- SSR genotyping of 200 tea (Camellia sinensis) clones obtained by selection and DNA barcoding of 12 varietal registration candidates.BMC plant biology · 2025Article
- Genetic Diversity and Population Structure of Wild AncientPlants (Basel, Switzerland) · 2025Article
- Rapid Identification of Alien Chromosome Fragments and Tracing of Bioactive Compound Genes in Intergeneric Hybrid Offspring BetweenInternational journal of molecular sciences · 2025Article
- Increasing jute (Frontiers in plant science · 2025Article
- Application of an Anchor Mapping of Alien Chromosome (AMAC) Fragment Localization Method in the Identification of Radish Chromosome Segments in the Progeny of Rape-Radish Interspecific Hybrids.International journal of molecular sciences · 2024Article
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8 authors.
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Abstract
The market value of tea is largely dependent on the tea species and cultivar. Therefore, it is important to develop efficient molecular markers covering the entire tea genome that can be used for the identification of tea varieties, marker-assisted breeding, and mapping important quantitative trait loci for beneficial traits. In this study, genome-wide molecular markers based on intron length polymorphism (ILP) were developed for tea trees. A total of 479, 1393, and 1342 tea ILP markers were identified using the PCR method in silico from the 'Shuchazao' scaffold genome, the chromosome-level genome of 'Longjing 43', and the ancient tea DASZ chromosome-level genome, respectively. A total of 230 tea ILP markers were used to amplify six tea tree species. Among these, 213 pairs of primers successfully characterize products in all six species, with 112 primer pairs exhibiting polymorphism. The polymorphism rate of primer pairs increased with the improvement in reference genome assembly quality level. The cross-species transferability analysis of 35 primer pairs of tea ILP markers showed an average amplification rate of 85.17% through 11 species in 6 families, with high transferability in
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