ArticleScience advances2024
Virtual formalin-fixed and paraffin-embedded staining of fresh brain tissue via stimulated Raman CycleGAN model.
Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 56 citations in OpenAlex.
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- PLANCK: super-multiplex optical imaging without labeling.bioRxiv : the preprint server for biology · 2026Article
- Dual-Modal Autofluorescence Microscopy for CycleGAN-Based H&E-like Image Generation in Liver Tissue: A Preliminary Proof-of-Concept Study.Diagnostics (Basel, Switzerland) · 2026Article
- AI-Assisted Coherent Raman Scattering Microscopy for Clinical Translation.Chemical & biomedical imaging · 2026Review
- Simultaneous dual-Raman-shift scanning-free stimulated Raman scattering microscopy for label-free biomolecular imaging.Nature communications · 2026Article
- Generative adversarial networks enable biomimetic topology fusion with balanced mechanical performance and aesthetic quality.Scientific reports · 2026Article
- His-MMDM: Multi-Domain and Multi-Omics Translation of Histopathological Images with Diffusion Models.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Label-free whole slide virtual multi-staining using dual-excitation photon absorption remote sensing microscopy.Npj imaging · 2026Article
- Real-Time In Vivo Cellular-Level Imaging During Puncture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Label-free intraoperative imaging of hemodynamics using deep learning.Biomedical optics express · 2026Article
- Real-time intraoperative confocal laser endomicroscopy with on-site neuropathologist consultation for glioma margin assessment: illustrative case.Journal of neurosurgery. Case lessons · 2026Article
- Label-free molecular profiling of cancer using Raman spectroscopy: from fundamentals to clinical applications.Frontiers in oncology · 2026Review
- Mid-infrared microscopy for label-free digital staining: initial clinical assessment.Frontiers in bioengineering and biotechnology · 2026Article
- DeepLuAd: Semantic-guided virtual histopathology of lung adenocarcinoma via stimulated Raman scattering.Theranostics · 2026Article
- Bond-Selective Imaging at the Frontier of Biomedicine.Chemical & biomedical imaging · 2025Article
- Bayesian reconstruction of rapidly scanned mid-infrared optoacoustic signals enables fast, label-free chemical microscopy.Science advances · 2025Article
- H&E Style Translation Using CycleGAN for Deep Ultraviolet-Excitation Fluorescence Images of Pancreatic Endoscopic Ultrasound-Fine Needle Aspiration/Biopsy Toward Slide-Free Rapid Pathology.Acta histochemica et cytochemica · 2025Article
- Fast and label-free 3D virtual H&E histology via active phase modulation-assisted dynamic full-field OCT.Npj imaging · 2025Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intraoperative histology is essential for surgical guidance and decision-making. However, frozen-sectioned hematoxylin and eosin (H&E) staining suffers from degraded accuracy, whereas the gold-standard formalin-fixed and paraffin-embedded (FFPE) H&E is too lengthy for intraoperative use. Stimulated Raman scattering (SRS) microscopy has shown rapid histology of brain tissue with lipid/protein contrast but is challenging to yield images identical to nucleic acid-/protein-based FFPE stains interpretable to pathologists. Here, we report the development of a semi-supervised stimulated Raman CycleGAN model to convert fresh-tissue SRS images to H&E stains using unpaired training data. Within 3 minutes, stimulated Raman virtual histology (SRVH) results that matched perfectly with true H&E could be generated. A blind validation indicated that board-certified neuropathologists are able to differentiate histologic subtypes of human glioma on SRVH but hardly on conventional SRS images. SRVH may provide intraoperative diagnosis superior to frozen H&E in both speed and accuracy, extendable to other types of solid tumors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.