ArticlePLoS genetics2024
Dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia.
Article in PLoS genetics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 30 citations in OpenAlex.
- Engineering monosex and sterile fish for food production: from conventional methods to CRISPR-based precision.Advanced biotechnology · 2026Review
- Achieving monosex male tilapia: a critical review of hormonal, biodegradable and genome editing strategies.Biologia futura · 2026Review
- Gonadal Transcriptomic Atlas Reveals Conserved and Sex-Biased Regulatory Networks in Black-Spotted Croaker (Protonibea diacanthus).Marine biotechnology (New York, N.Y.) · 2026Article
- Integrated Analysis of mRNA-seq and miRNA-seq Reveal the Dynamics of the Gonadal Differentiation of Procypris Mera.Marine biotechnology (New York, N.Y.) · 2026Article
- RNA interference-mediated silencing of amh reveals its regulatory role in gonadal development and sex hormone synthesis in grass carp (Ctenopharyngodon idella).Fish physiology and biochemistry · 2026Article
- Single-nucleus RNA sequencing reveals the underlying roadmap of early gonadal differentiation in teleost.Science China. Life sciences · 2026Article
- A Multi-omics Atlas of Gonadal Sex Differences and Characterization of the dmrt1 Gene in Gymnocypris Eckloni Herzenstein.Marine biotechnology (New York, N.Y.) · 2026Article
- Comparative RNA-Seq Analysis of Differentially Expressed Genes in the Testis and Ovary of Mudskipper,Animals : an open access journal from MDPI · 2026Article
- Evaluation of an Infertile, All-Male ZZ Line Exhibiting Female-like Growth in Chinese Tongue Sole (Biology · 2026Article
- Transposable Element-Mediated Cis-Regulation Drives the Evolution of dmrt1 as a Candidate Master Sex-Determining Gene in Black Carp.Molecular biology and evolution · 2025Article
- Long Non-Coding RNAInternational journal of molecular sciences · 2025Article
- Early Temporal Dynamics of Gonadal Development and Sexually Dimorphic Gene Expression During Sex Differentiation in Mandarin Fish (Siniperca chuatsi).Marine biotechnology (New York, N.Y.) · 2025Article
- Dynamic Chromatin Accessibility and Transcriptional Regulation in the Eyes of Red Tilapia (Oreochromis sp.) in Response to Wintering Stress.Marine biotechnology (New York, N.Y.) · 2025Article
- Sexual Dimorphic Expression ofSexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2025Article
- Article
- Comparable to 17α- methyl testosterone, dietary supplements of Tribulus terrestris and Mucuna pruriens promote the development of mono-sex, all-male tilapia fry, growth, survival rate and sex-related genes (Amh, Sox9, Foxl2, Dmrt1).BMC veterinary research · 2024Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sex is determined by multiple factors derived from somatic and germ cells in vertebrates. We have identified amhy, dmrt1, gsdf as male and foxl2, foxl3, cyp19a1a as female sex determination pathway genes in Nile tilapia. However, the relationship among these genes is largely unclear. Here, we found that the gonads of dmrt1;cyp19a1a double mutants developed as ovaries or underdeveloped testes with no germ cells irrespective of their genetic sex. In addition, the gonads of dmrt1;cyp19a1a;cyp19a1b triple mutants still developed as ovaries. The gonads of foxl3;cyp19a1a double mutants developed as testes, while the gonads of dmrt1;cyp19a1a;foxl3 triple mutants eventually developed as ovaries. In contrast, the gonads of amhy;cyp19a1a, gsdf;cyp19a1a, amhy;foxl2, gsdf;foxl2 double and amhy;cyp19a1a;cyp19a1b, gsdf;cyp19a1a;cyp19a1b triple mutants developed as testes with spermatogenesis via up-regulation of dmrt1 in both somatic and germ cells. The gonads of amhy;foxl3 and gsdf;foxl3 double mutants developed as ovaries but with germ cells in spermatogenesis due to up-regulation of dmrt1. Taking the respective ovary and underdeveloped testis of dmrt1;foxl3 and dmrt1;foxl2 double mutants reported previously into consideration, we demonstrated that once dmrt1 mutated, the gonad could not be rescued to functional testis by mutating any female pathway gene. The sex reversal caused by mutation of male pathway genes other than dmrt1, including its upstream amhy and downstream gsdf, could be rescued by mutating female pathway gene. Overall, our data suggested that dmrt1 is the only male pathway gene tested indispensable for sex determination and functional testis development in tilapia.
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