Evidence map›Paper›PMID 38532097›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Phenol-Based Protein Extraction Method for Plant Proteomic Studies.

Przemysław Kopeć, Monika Krzewska, Agnieszka Płażek

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 90% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 0 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 1 country.

Przemysław KopećThe Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland. p.kopec@ifr-pan.edu.pl.
Monika KrzewskaThe Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, Kraków, Poland.
Agnieszka PłażekDepartment of Physiology, Breeding of Plant and Seed Science, University of Agriculture, Cracow, Poland.
Franciszek Górski Institute of Plant Physiology · PLPolish Academy of Sciences · PLUniversity of Agriculture in Krakow · PL

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The protein extraction method based on the phenol solution and combined with protein precipitation with ammonium acetate in methanol and purification in the same solution, and additionally in acetone and ethanol, is recommended for proteomic studies of plant tissues. The obtained protein samples do not require additional nucleic acid digestion and removal of interfering contaminations. The presented protocol was used to analyze the proteome of common buckwheat flowers and leaves.

Indexed as

PhenolPlant ProteinsElectrophoresis, Gel, Two-DimensionalPhenolsPlant LeavesPlantsProteomicsPhenolPhenolsPlant ProteinsPhenol extractionProtein precipitationProtein purificationProtein solubilizationProteome

Identifiers

PMID38532097
OpenAlexW4393206968

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.