ReviewCommunications biology2024
More than double the fun with two-photon excitation microscopy.
Review in Communications biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 57 citations in OpenAlex.
- Two-Photon Fluorescence and Second-Harmonic Generation Microscopy: Principles and Biomedical Applications.Journal of biophotonics · 2026Review
- Submicromolar imaging of intrinsic chromophores by two-photon photothermal microscopy captures mitochondrial response to chemotherapy.Science advances · 2026Article
- Thioflavin-T derivatives with controlled AIE and ACQ properties as potential one-photon and two-photon amyloid markers.Scientific reports · 2026Article
- Article
- Live imaging of vascular dynamics in mouse skin.The Journal of investigative dermatology · 2026Article
- Deep Learning Integration in Optical Microscopy: Advancements and Applications.Microscopy research and technique · 2026Review
- Serial intravital microscopy reveals temporal dynamics of autoreactive germinal centers in the spleen.iScience · 2026Article
- Amplitude, relative phase, and polarization characterization during ultrashort pulse of hybrid mode-locked thulium-doped all-fiber laser.Scientific reports · 2026Article
- Spectroscopic Super-resolution Imaging of DNA Molecules using Intrinsic Contrast.Journal of visualized experiments : JoVE · 2026Article
- A dual-color miniature endoscope for calcium imaging in behaving mice.bioRxiv : the preprint server for biology · 2026Article
- Upconversion Photoluminescence to Monitor Local Heat Release During Femtosecond Direct Laser Writing of Bioinks In Situ.Small methods · 2026Article
- OPTRACE: Optical Imaging-Guided Transplantation and Tracking of Cells in the Mouse Brain.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Advanced imaging strategies in cardiac organoids: bridging the gap between structural complexity and functional analysis.Cardiovascular diabetology · 2026Review
- Fully 3D-printed endomicroscopic objective for two-photon, multi-wavelength excitation microscopy.Biomedical optics express · 2026Article
- Evaluation of a nanosecond dissipative soliton resonance fiber laser for multiphoton microscopy.Scientific reports · 2025Article
- Quantitative cytoarchitectural phenotyping of deparaffinized human brain tissues.Communications biology · 2025Article
- Descattering and image restoration with a transformer-based neural network in deep tissue imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Two-Photon Absorption and Dynamics of Excited States in Bromochalcone Derivatives.The journal of physical chemistry. A · 2025Article
- Tissue type and tissue depth dependency of pulse broadening in three-photon microscopy: impact on third harmonic generation.Biomedical optics express · 2025Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
For generations researchers have been observing the dynamic processes of life through the lens of a microscope. This has offered tremendous insights into biological phenomena that span multiple orders of time- and length-scales ranging from the pure magic of molecular reorganization at the membrane of immune cells, to cell migration and differentiation during development or wound healing. Standard fluorescence microscopy techniques offer glimpses at such processes in vitro, however, when applied in intact systems, they are challenged by reduced signal strengths and signal-to-noise ratios that result from deeper imaging. As a remedy, two-photon excitation (TPE) microscopy takes a special place, because it allows us to investigate processes in vivo, in their natural environment, even in a living animal. Here, we review the fundamental principles underlying TPE aimed at basic and advanced microscopy users interested in adopting TPE for intravital imaging. We focus on applications in neurobiology, present current trends towards faster, wider and deeper imaging, discuss the combination with photon counting technologies for metabolic imaging and spectroscopy, as well as highlight outstanding issues and drawbacks in development and application of these methodologies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.