Evidence map›Paper›PMID 38531188›Full record

ReviewCurrent opinion in structural biology2024

Modern approaches to improving phase contrast electron microscopy.

Jeremy J Axelrod, Jessie T Zhang, Petar N Petrov, Robert M Glaeser, Holger Müller

Abstract readReview
PubMed Publisher
In one paragraph

Review in Current opinion in structural biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Article
  5. Review
  6. Article
  7. Review
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  9. Article
  10. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Jeremy J AxelrodDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA; Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA.
Jessie T ZhangDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA.
Petar N PetrovDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA; Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA.
Robert M GlaeserDepartment of Molecular and Cell Biology, University of California Berkeley, Berkeley, CA 94720, USA.
Holger MüllerDepartment of Physics, University of California Berkeley, Berkeley, CA 94720, USA; Lawrence Berkeley National Laboratory, One Cyclotron Road, Berkeley, CA 94720, USA. Electronic address: hm@berkeley.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although defocus can be used to generate partial phase contrast in transmission electron microscope images, cryo-electron microscopy (cryo-EM) can be further improved by the development of phase plates which increase contrast by applying a phase shift to the unscattered part of the electron beam. Many approaches have been investigated, including the ponderomotive interaction between light and electrons. We review the recent successes achieved with this method in high-resolution, single-particle cryo-EM. We also review the status of using pulsed or near-field enhanced laser light as alternatives, along with approaches that use scanning transmission electron microscopy (STEM) with a segmented detector rather than a phase plate.

Indexed as

Cryoelectron MicroscopyMicroscopy, Phase-Contrast

Identifiers

PMID38531188

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.