Evidence map›Paper›PMID 38526793›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Fluorescence Lifetime Measurements and Analyses: Protocols Using Flow Cytometry and High-Throughput Microscopy.

Jessica P Houston, Samantha Valentino, Aric Bitton

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Jessica P HoustonDepartment of Chemical & Materials Engineering, New Mexico State University, Las Cruces, NM, USA. jph@nmsu.edu.
Samantha ValentinoDepartment of Chemical & Materials Engineering, New Mexico State University, Las Cruces, NM, USA.
Aric BittonCytek Biosciences, Fremont, CA, USA.

Funding

Advanced approaches for biomedical measurements using time-resolved and micro-flow cytometryR35GM152076 · NIGMS · NEW MEXICO STATE UNIVERSITY LAS CRUCES · PI Jessica Perea Houston · 2024 to 2026
$1.1M
NIGMS NIH HHS R35 GM152076
6 · The paper itself

Abstract

This chapter focuses on applications and protocols that involve the measurement of the fluorescence lifetime as an informative cytometric parameter. The timing of fluorescence decay has been well-studied for cell counting, sorting, and imaging. Therefore, provided herein is an overview of the techniques used, how they enhance cytometry protocols, and the modern techniques used for lifetime analysis. The background and theory behind fluorescence decay kinetic measurements in cells is first discussed followed by the history of the development of time-resolved flow cytometry. These sections are followed by a review of applications that benefit from the quantitative nature of fluorescence lifetimes as a photophysical trait. Lastly, perspectives on the modern ways in which the fluorescence lifetime is scanned at high throughputs which include high-speed microscopy and machine learning are provided.

Indexed as

Fluorescent DyesReview Literature as TopicFlow CytometryFluorescenceKineticsMicroscopy, FluorescenceFluorescent DyesFluorescence lifetimeFrequency domainFRETTime domainTime-resolved flow cytometry

Identifiers

PMID38526793

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.