Evidence map›Paper›PMID 38526781›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2024

Laser Sources for Traditional and Spectral Flow Cytometry.

William G Telford

Abstract readReview
PubMed Publisher
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Spectral Flow Cytometry: The Current State and Future of the Technology.International journal of molecular sciences · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

William G TelfordLaboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA. telfordw@mail.nih.gov.

Funding

FACS Core FacilityZICBC011138 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI TELFORD, WILLIAM · 2009 to 2025
$14.5M
6 · The paper itself

Abstract

Lasers for light scattering measurement and fluorescence excitation are essential components of all flow cytometers. Flow cytometers now typically rely on multiple laser wavelengths allowing excitation of a constantly increasing variety of fluorescent probes. The expanding use of spectral flow cytometry to increase the magnitude of multiparametric analysis is also changing the significance of laser choice in cytometry. In this chapter, we review the lasers available for flow cytometry and provide guidance in choosing laser wavelengths and characteristics to best match the needs of modern cell analysis by both conventional and spectral cytometry. We also discuss the recent advances in laser technology as the push to expand the palette of laser wavelength for cytometry continues.

Indexed as

LasersLightFlow CytometryFluorescent DyesNephelometry and TurbidimetryFluorescent DyesDeep ultravioletdiodeDiode-pumped solid-state laserFlow cytometryInfraredLaserLaser engineMerge moduleOptically pumped semiconductor laserSupercontinuum

Identifiers

PMID38526781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.